Cytochrome P450 Protocols - Phillips, Ian R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
Cytochrome P450 Nomenclature, 2004 -- Spectral Analyses of Cytochromes P450 -- Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli -- Purification of Cytochromes P450 -- Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System -- Cytochrome P450 Reconstitution Systems -- Catalytic Assays for Human Cytochrome P450 -- Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate -- Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation -- Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes -- High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6?-Hydroxylation -- Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography -- CYP2C19-Mediated (S)-Mephenytoin 4?-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection -- CYP2D6-Dependent Bufuralol 1?-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection -- Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation -- Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation -- Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection -- An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7?-Hydroxylation -- Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families -- Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity -- Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases -- Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes -- The Human Cytochrome P450 Allele Nomenclature Committee Web Site -- Genotyping for Cytochrome P450 Polymorphisms -- Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing -- Isolation of Rat Hepatocytes -- Rat Hepatocyte Cultures -- Rat Hepatocyte Cultures -- Hepatocytes in Suspension -- Isolation of Rat Bone Marrow Stem Cells -- Transfection of Primary Cultures of Rat Hepatocytes -- Human Hepatocyte Culture -- Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization -- Deletion of Genes From the Mouse Genome Using Cre/loxP Technology -- Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes -- Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes -- Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription.
Keywords: Biochemistry; Biochemistry, general