Directed Enzyme Evolution : Screening and Selection Methods - Arnold, Frances H.; Georgiou, George; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2003)
Genetic Selections -- Genetic Complementation Protocols -- Use of Pol I-Deficient E. coli for Functional Complementation of DNA Polymerase -- Selection of Novel Eukaryotic DNA Polymerases by Mutagenesis and Genetic Complementation of Yeast -- Autogene Selections -- Selection for Soluble Proteins via Fusion with Chloramphenicol Acetyltransferase -- Proside -- Minimization of Proteins by Random Fragmentation and Selection -- Screens for Enzymes -- Evaluating a Screen and Analysis of Mutant Libraries -- Screening Mutant Libraries in Saccharomyces cerevisiae -- Solid-Phase Screening Using Digital Image Analysis -- Screening for Thermostability -- High-Throughput Screening of Mutant ?-Amylase Libraries for Increased Activity at 129°C -- High-Throughput Carbon Monoxide Binding Assay for Cytochromes P450 -- High-Throughput Screen for Aromatic Hydroxylation -- Colorimetric Screen for Aliphatic Hydroxylation by Cytochrome P450 Using p-Nitrophenyl-Substituted Alkanes -- High-Throughput Screens Based on NAD(P)H Depletion -- High-Throughput Tetramethylbenzidine (TMB) Screen for Peroxidases -- Screen for Oxidases by Detection of Hydrogen Peroxide with Horseradish Peroxidase -- Colorimetric Dehydrogenase Screen Based on NAD(P)H Generation -- Colorimetric Assays for Screening Laccases -- pH Sensing Agar Plate Assays for Esterolytic Enzyme Activity -- A pH-Indicator-Based Screen for Hydrolytic Haloalkane Dehalogenase -- Detection of Aromatic ?-Hydroxyketones with Tetrazolium Salts -- Selection of Heat-Stable Clostridium cellulovorans Cellulases After In Vitro Recombination -- Screening and Selection Strategies for Disulfide Isomerase Activity -- An Overview of High-Throughput Screening Systems for Enantioselective Enzymatic Transformations -- Select Protocols of High-Throughput ee-Screening Systems for Assaying Enantioselective Enzymes -- Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell Sorting (FACS) -- Calmodulin-Tagged Phage and Two-Filter Sandwich Assays for the Identification of Enzymatic Activities -- High-Throughput FACS Method for Directed Evolution of Substrate Specificity -- Improving Protein Folding Efficiency by Directed Evolution Using the GFP Folding Reporter.
Keywords: Cell biology; Cell Biology