Expressed Sequence Tags (ESTs) : Generation and Analysis - Parkinson, John.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Expressed Sequence Tags: An Overview -- Strategies for Undertaking Expressed Sequence Tag (EST) Projects -- Construction of cDNA Libraries: Focus on Protists and Fungi -- Generation of Full-Length cDNA Libraries: Focus on Plants -- Preparation of Full-Length cDNA Libraries: Focus on Metazoans -- Expressed Sequence Tags: Normalization and Subtraction of cDNA Libraries Expressed sequence tags normalization and subtraction of cDNA libraries -- Generating EST Libraries: Trans-Spliced cDNAs -- Large-Scale Sequencing and Analytical Processing of ESTs -- EST Processing: From Trace to Sequence -- Obtaining Accurate Translations from Expressed Sequence Tags -- EST Databases and Web Tools for EST Projects -- Phylogenomic Analysis of EST Datasets -- Statistical Analysis of Expressed Sequence Tags -- Application of ESTs in Microarray Analysis -- Exploiting ESTs in Human Health.
Keywords: Biochemistry; Human genetics; Medical genetics; Biochemistry, general; Human Genetics; Gene Function