Glycoprotein Methods and Protocols : The Mucins - Corfield, Anthony P.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2000)
Purification of Mucin -- Isolation of Large Gel-Forming Mucins -- Preparation of Membrane Mucin -- Detection and Ouantitation of Mucin -- Histologically Based Methods for Detection of Mucin -- Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods -- The Gastrointestinal Adherent Mucous Gel Barrier -- Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling -- Separation, Identification, and Physical Characterization of Mucins -- Separation and Identification of Mucins and Their Glycoforms -- Heterogeneity and Size Distribution of Gel-Forming Mucins -- Rheology of Mucin -- Rheology of Mucin -- Mucin Peptide Analysis -- Amino Acid Analysis of Mucins -- Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation -- Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies -- Mucin Domains to Explore Disulfide-Dependent Dimer Formation -- Carbohydrate Structural Analysis of Mucins -- Monosaccharide Composition of Mucins -- O-Linked Oligosaccharide Chain Release and Fractionation -- Structural Analysis of Mucin-Type O-Linked Oligosaccharides -- Measurement of Sulfate in Mucins -- Mucin Biosynthesis -- Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study -- Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin -- Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis -- Mucin Precursors Identification and Analysis of Their Intracellular Processing -- Inhibition of Mucin Glycosylation -- O-Linked Chain Glycosyltransferases -- Mucin Gene Detection -- Mucin cDNA Cloning -- Northern Blot Analysis of Large mRNAs -- Southern Blot Analysis of Large DNA Fragments -- In Situ Hybridization Techniques for Localizing Mucin mRNA -- Detection of Mucin Gene Polymorphism -- Preparation of Antimucin Antibodies -- Polyclonal and Monoclonal Techniques -- Monoclonal Antibodies to Mucin VNTR Peptides -- Enzymatic Degradation of Mucins -- Mucinase Activity -- Proteinase Activity -- Glycosidase Activity -- Assays for Bacterial Mucin-Desulfating Sulfatases -- Mucin-Bacterial Interactions -- Mucin-Bacterial Binding Assays -- Growth of Mucin Degrading Bacteria in Biofilms -- Cellular And Humoral Responses To Mucins -- Generation of MUC1 CytotoxicT-Cells in Mice and Epitope Mapping -- Analysis of the Frequency of MHC-Unrestricted MUC1 -Specific CytotoxicT-Cells in Peripheral Blood by Limiting Dilution Assay -- Expression of MUC1 in Insect Cells Using Recombinant Baculovirus -- Mucin Antigen Presentation Using Dendritic Cells -- Detection of Humoral Immune Responses to Mucins.
Keywords: Biochemistry; Biochemistry, general