Cystic Fibrosis Methods and Protocols - Skach, William R.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Genetics of Cystic Fibrosis -- CFTR Mutation Detection by Multiplex Heteroduplex (mHET) Analysis on MDE Gel -- cDNA Microarrays for Pharmacogenomic Analysis of Cystic Fibrosis -- Natural Animal Models of Human Genetic Diseases -- CFTR structure and Function: -- Electrophysiological Approach to Studying CFTR -- Quantitative Analysis of ATP-Dependent Gating of CFTR -- CFTR Regulation by Phosphorylation -- Transepithelial Measurements of Bicarbonate Secretion in Calu-3 Cells -- Transepithelial Impedance Analysis of Chloride Secretion -- Studies of the Molecular Basis for Cystic Fibrosis Using Purified Reconstituted CFTR Protein -- Probing CFTR Channel Structure and Function Using the Substituted-Cysteine-Accessibility Method -- Methods for the Study of Intermolecular and Intramolecular Interactions Regulating CFTR Function -- Fluorescent Indicator Methods to Assay Functional CFTR Expression in Cells -- Immunolocalization of CFTR in Intact Tissue and Cultured Cells -- Analysis of CFTR Trafficking and Polarization Using Green Fluorescent Protein and Confocal Microscopy -- CFTR Folding and Maturation in Cells -- Isolation of CFTR -- CFTR Expression and ER-Associated Degradation in Yeast -- Manipulating the Folding Pathway of ?F508 CFTR Using Chemical Chaperones -- CFTR Degradation and Aggregation -- In Vitro Reconstitution of CFTR Biogenesis and Degradation -- In Vitro CFTR Folding Assays -- Analysis of CFTR Endocytosis by Cell Surface Biotinylation -- CFTR Regulation of ENaC -- Yeast Two-Hybrid Identification and Analysis of Protein Interactions with CFTR -- Biochemical Assays for Studying Indirect Interactions Between CFTR and the Cytoskeleton -- CFTR-Associated ATP Transport and Release -- Pathophysiology of Cystic Fibrosis -- Inflammatory Mediators in CF Patients -- Bacterial Colonization and Infection in the CF Lung -- Antimicrobial Peptides and Proteins in the CF Airway -- Bacterial-Epithelial Interactions -- Thin-Film Measurements of Airway Surface Liquid Volume/Composition and Mucus Transport Rates In Vitro -- Murine Models of CF Airway Infection and Inflammation -- Analysis of Lipid Abnormalities in CF Mice -- Bioelectric Measurement of CFTR Function in Mice -- Xenograft Model of the CF Airway -- Development of Conditionally Immortalized Epithelial Cell Lines from CF and Non-CF Mice -- Technical Approaches to Analyze the In Vivo Ion Composition of Airway Surface Liquid -- Novel Therapeutic Approaches for Cystic Fibrosis -- Design of Gene Therapy Clinical Trials in CF Patients -- Formulation of Synthetic Vectors for Cystic Fibrosis Gene Therapy -- Adeno-Associated Viral Vectors for CF Gene Therapy.
Keywords: Respiratory organs-Diseases.; Pneumology/Respiratory System