Cytoskeleton Methods and Protocols - Gavin, Ray H.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Identification of Cytoskeleton Proteins -- Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments -- Microsequencing of Myosins for PCR Primer Design -- Evaluating the Dynein Heavy Chain Gene Family in Tetrahymena -- Microscopy Applications -- Video-Enhanced Microscopy for Analysis of Cytoskeleton Structure and Function -- Computer-Assisted Systems for the Analysis of Amoeboid Cell Motility -- Evaluation of Individual-Cell Motility -- Evaluation of Cell Morphology -- A Quantitative Assay for Measurement of Chemokinesis in Tetrahymena -- Reagents for Studying Cytoskeleton Protein Function -- Jasplakinolide -- Site-Directed Antibodies as Tools for Investigating Structure and Function of Cytoskeleton Proteins -- Direct Labeling of Components in Protein Complexes by Immuno-Electron Microscopy -- Tat-Mediated Delivery of Antibodies into Cultured Cells -- Cytoskeleton Dynamics -- Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein -- Use of Green Fluorescent Protein (GFP) to Study Cellular Dynamics -- Transient Transfections and Heterokaryons as Tools for the Analysis of Keratin IF Dynamics -- Cellular Systems as Tools for Investigating Cytoskeleton Structure and Function -- Chromatophores as Tools for the Study of Organelle Transport -- Centriole Duplication, Centrosome Maturation and Spindle Assembly in Lysates of Spisula solidissima Oocytes -- XenopusEgg Extracts as a Model System for Analysis of Microtubule, Actin Filament, and Intermediate Filament Interactions -- Detergent-Extracted Models for the Study of Cilia or Flagella -- Genetic Approaches for Studies of Cytoskeleton Protein Function -- A Yeast Two-Hybrid Approach for Probing Cytoskeletal Protein Interactions -- Manipulating Dynein Genes in Tetrahymena -- Functional Analysis of Cytoskeletal Components in the Developing Visual System of Drosophila.
Keywords: Cell biology; Cell Biology