Epigenome Editing : Methods and Protocols - Jeltsch, Albert; Rots, Marianne G.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Editing the Epigenome: Overview, Open Questions, and Directions of Future Development -- Zinc Fingers, TALEs and CRISPR Systems: A Comparison of Tools for Epigenome Editing -- Designing Epigenome Editors: Considerations of Biochemical and Locus Specificities -- Generation of TALE-Based Designer Epigenome Modifiers -- Neuroepigenetic Editing -- Allele-Specific Epigenome Editing -- Key to Delivery: The (Epi-)Genome Editing Vector Toolbox -- CRISPR/dCas9 Switch Systems for Temporal Transcriptional Control -- Delivery of Designer Epigenome Modifiers into Primary Human T Cells -- Viral Expression of Epigenome Editing Tools in Rodent Brain Using Stereotaxic Surgery Techniques -- Stable Expression of Epigenome Editors Via Viral Delivery and Genomic Integration -- Purified-Protein Delivery to Activate an Epigenetically-Silenced Allele in Mouse Brain -- Non-Viral Methodology for Efficient Co-Transfection -- Chromatin Immunoprecipitation in Human and Yeast Cells -- Chromatin Immunoprecipitation and High Throughput Sequencing (ChIP-Seq): Tips and Tricks Regarding the Laboratory Protocol and Initial Downstream Data Analysis -- Generation of Whole Genome Bisulfite Sequencing Libraries for Comprehensive DNA Methylome Analysis -- Approaches for the Analysis and Interpretation of Whole Genome Bisulfite Sequencing Data -- Whole Genome Bisulfite Sequencing for the Analysis of Genome-Wide DNA Methylation and Hydroxymethylation Patterns at Single-Nucleotide Resolution -- Locus-Specific DNA Methylation Analysis by Targeted Deep Bisulfite Sequencing -- DNA Methylation Analysis by Bisulfite Conversion Coupled to Double Multiplexed Amplicon-Based Next-Generation Sequencing (NGS) -- Cell-to-Cell Transcription Variability as Measured by Single Molecule RNA FISH to Detect Epigenetic State Switching -- Establishment of Cell Lines Stably Expressing dCas-Fusions to Address Kinetics of Epigenetic Editing -- Editing of DNA Methylation Using dCas9-Peptide Repeat and scFv-TET1 Catalytic Domain Fusions -- Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells -- Screening Regulatory Element Function with CRISPR/Cas9-Based Epigenome Editing.
Keywords: Human genetics; Human Genetics