Epidermal Cells : Methods and Protocols - Turksen, Kursad; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2005)
Keratinocyte and Organ Cultures -- Primary Mouse Keratinocyte Culture -- Serial Cultivation of Primary Adult Murine Keratinocytes -- Keratinocyte Culture in the Absence of Substrate Attachment -- Application of Genetically Modified Feeder Cells for Culture of Keratinocytes -- Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis -- Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo -- In Vitro Fabrication of Engineered Human Skin -- Epidermal Stem Cells -- In Vivo Labeling and Analysis of Epidermal Stem Cells -- Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice -- FACS Enrichment of Human Keratinocyte Stem Cells -- Isolation, Characterization, and Culture of Epithelial Stem Cells -- Keratin 19 as a Stem Cell Marker In Vivo and In Vitro -- Analysis of Epidermal Differentiation -- Immunolocalization in the Epidermis -- Epidermal Cell Analysis by RT-PCR -- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis -- Analysis of Early Epidermal Development in Zebrafish -- Analysis of E2F Factors During Epidermal Differentiation -- Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis -- Apoptosis in the Epidermis -- Fate of Desmosomal Proteins in Apoptotic Epidermal Cells -- Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry -- MMP-9 and TIMP-1 Assays in Keratinocyte Cultures -- Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins -- Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval -- Methods and Approaches for the Analysis for Epidermal Function -- Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods -- Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry -- Fluorimetric DNA Assay of Cell Number -- Keratinocyte Transient Transfections -- Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes -- Gene Targeting by Oligonucleotides in Keratinocytes -- Promoter Analysis in the Human SPRR Gene Family -- Stable Integration of Large PAC Constructs in Keratinocytes -- Targeted Somatic Mutagenesis in the Mouse Epidermis -- Methods to Study Protein-Protein Interactions -- Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes -- Analysis of Tissue-Specific DNA Methylation During Development -- Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis -- Methods for Gene Expression Profiling in Dermatology Research Using DermArray® Nylon Filter DNA Microarrays -- Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis -- Transplantation and Gene Therapy -- In Vivo Transplantation of Engineered Human Skin -- Epidermis-Targeted Gene Transfer Using In Vivo Electroporation -- Gene and Stem Cell Therapy of the Hair Follicle.
Keywords: Internal medicine; Cell biology; Internal Medicine; Cell Biology