DNA-Protein Interactions : Principles and Protocols, Third Edition - Moss, Tom.; Leblanc, Benoît.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Filter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Exonuclease III Footprinting on Immobilized DNA Templates -- Hydroxyl Radical Footprinting of Protein-DNA Complexes -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Uranyl Photofootprinting -- Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference -- Ethylation Interference Footprinting of DNA-Protein Complexes -- Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates Within Model Chromatin Complexes -- Identification of Nucleic Acid High-Affinity Binding Sequences of Proteins by SELEX -- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Determination of a Transcription Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Use of a Reporter Gene Assay in Yeast for Genetic Analysis of DNA-Protein Interactions -- Chromatin Immunoprecipitation in Mammalian Cells -- Sequential Chromatin Immunoprecipitation Protocol: ChIP-reChIP -- Profiling Genome-Wide Histone Modifications and Variants by ChIP-chip on Tiling Microarrays in S. cerevisiae -- Nucleosome Mapping -- In Cellulo DNA Analysis (LMPCR Footprinting) -- Atomic Force Microscopy Imaging and Probing of DNA, Proteins, and Protein-DNA Complexes: Silatrane Surface Chemistry -- Two-Dimensional Crystallisation of Soluble Protein Complexes -- Assays for Transcription Factor Activity -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Static and Kinetic Site-Specific Protein-DNA Photocrosslinking: Analysis of Bacterial Transcription Initiation Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking of Purified Complexes to Analyze the Topology of the RNA Polymerase II Transcription Initiation Complex -- Site-Directed DNA Crosslinking of Large Multisubunit Protein-DNA Complexes -- Functional Studies of DNA-Protein Interactions Using FRET Techniques -- Single-Molecule FRET Analysis of Protein-DNA Complexes -- Analysis of DNA Supercoiling Induced by DNA-Protein Interactions -- The Cruciform DNA Mobility Shift Assay: A Tool to Study Proteins that Recognize Bent DNA -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Analysis of Distant Communication on Defined Chromatin Templates In Vitro -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Fluorescence Spectroscopy and Anisotropy in the Analysis of DNA-Protein Interactions -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Defining the Thermodynamics of Protein/DNA Complexes and Their Components Using Micro-calorimetry -- Surface Plasmon Resonance Assays of DNA-Protein Interactions.
Keywords: Proteins .; Human genetics; Proteomics; Protein Science; Human Genetics; Proteomics