Genetic Modification of Hematopoietic Stem Cells : Methods and Protocols - Baum, Christopher; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications -- Isolation of Human and Mouse Hematopoietic Stem Cells -- Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors -- Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials -- Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer -- T Cell Culture for Gammaretroviral Transfer -- Retroviral Transduction of Murine Primary T Lymphocytes -- Lentiviral Vector Gene Transfer into Human T Cells -- DNA Transposons for Modification of Human Primary T Lymphocytes -- Retroviral Gene Transfer into Primary Human Natural Killer Cells -- Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells -- In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells -- In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors -- Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells -- Knock-Down of Gene Expression in Hematopoietic Cells -- The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations -- Detection of Replication Competent Retrovirus and Lentivirus -- Release Testing of Retroviral Vectors and Gene-Modified Cells -- Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells -- Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection -- Leukemia Diagnosis in Murine Bone Marrow Transplantation Models -- Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment -- Canine Models of Gene-Modified Hematopoiesis -- Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples -- Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones -- Tracking Gene-Modified T Cells In Vivo -- DNA Microarray Studies of Hematopoietic Subpopulations -- Quantification of Genomic Mutations in Murine Hematopoietic Cells -- Proteomics Studies After Hematopoietic Stem Cell Transplantation -- Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells -- Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis -- The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors.
Keywords: Biochemistry; Cell biology; Stem cells; Gene therapy; Biochemistry, general; Cell Biology; Stem Cells; Gene Therapy