Embryonic Stem Cells : Methods and Protocols - Turksen, Kursad; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2002)
Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells -- The Use of Chemically Defined Media for the Analyses of Early Development in ES Cells and Mouse Embryos -- Analysis of the Cell Cycle in Mouse Embryonic Stem Cells -- Murine Embryonic Stem Cells as a Model for Stress Proteins and Apoptosis During Differentiation -- Effects of Altered Gene Expression on ES Cell Differentiation -- Hypoxic Gene Regulation in Differentiating ES Cells -- Regulation of Gap Junction Protein (Connexin) Genes and Function in Differentiating ES Cells -- Embryonic Stem Cell Differentiation as a Model to Study Hematopoietic and Endothelial Cell Development -- Analysis of Bcr-Abl Function Using an In Vitro Embryonic Stem Cell Differentiation System -- Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development -- Differentiation of Embryonic Stem Cells as a Model to Study Gene Function During the Development of Adipose Cells -- Embryonic Stem Cell Differentiation and the Vascular Lineage -- Embryonic Stem Cells as a Model to Study Cardiac, Skeletal Muscle, and Vascular Smooth Muscle Cell Differentiation -- Cardiomyocyte Enrichment in Differentiating ES Cell Cultures: Strategies and Applications -- Embryonic Stem Cells as a Model for the Physiological Analysis of the Cardiovascular System -- Isolation of Lineage-Restricted Neural Precursors from Cultured ES Cells -- Lineage Selection for Generation and Amplification of Neural Precursor Cells -- Selective Neural Induction from ES Cells by Stromal Cell-Derived Inducing Activity and Its Potential Therapeutic Application in Parkinson's Disease -- Epidermal Lineage -- ES Cell Differentiation Into the Hair Follicle Lineage In Vitro -- Embryonic Stem Cells as a Model for Studying Melanocyte Development -- Using Progenitor Cells and Gene Chips to Define Genetic Pathways -- ES Cell-Mediated Conditional Transgenesis -- Switching on Lineage Tracers Using Site-Specific Recombination -- From ES Cells to Mice: The Gene Trap Approach -- Functional Genomics by Gene-Trapping in Embryonic Stem Cells -- Phage-Displayed Antibodies to Detect Cell Markers -- Gene Transfer Using Targeted Filamentous Bacteriophage -- Single-Cell PCR Methods for Studying Stem Cells and Progenitors -- Nonradioactive Labeling and Detection of mRNAs Hybridized onto Nucleic Acid cDNA Arrays -- Expression Profiling Using Quantitative Hybridization on Macroarrays -- Isolation of Antigen-Specific Intracellular Antibody Fragments as Single Chain Fv for Use in Mammalian Cells -- Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays -- Direct Selection of cDNAs by Phage Display -- Screening for Protein-Protein Interactions in the Yeast Two-Hybrid System in Embryonic Stem Cells.
Keywords: Cell biology; Cell Biology