Enzyme Engineering : Methods and Protocols - Samuelson, James C.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2013)
A Tripartite Fusion System for the Selection of Protein Variants with Increased Stability in vivo -- Determining Enzyme Kinetics via Isothermal Titration Calorimetry -- GFP Reporter Screens for the Engineering of Amino Acid Degrading Enzymes from Libraries Expressed in Bacteria -- Flow Cytometric Assays for Interrogating LAGLIDADG Homing Endonuclease DNA Binding and Cleavage Properties -- TAL Effector Nuclease (TALEN) Engineering -- In vitro Evolution of Enzymes -- Residue-Specific Incorporation of Unnatural Amino Acids into Proteins In vitro and In vivo -- Reconstructing Evolutionary Adaptive Paths for Protein Engineering -- Oligonucleotide Recombination Enabled Site-Specific Mutagenesis in Bacteria -- FX Cloning: A Versatile High-Throughput Cloning System for Characterization of Enzyme Variants -- Use of Sulfolobus solfataricus PCNA Subunit Proteins to Direct the Assembly of Multimeric Enzyme Complexes -- Gene Synthesis by Assembly of Deoxyuridine Containing Oligonucleotides -- Protein Engineering: Single or Multiple Site-Directed Mutagenesis -- Gene Assembly and Combinatorial Libraries in S. cerevisiae via Reiterative Recombination -- Promiscuity-Based Enzyme Selection for Rational Directed Evolution Experiments -- Rational Protein Sequence Diversification by Multi-Codon Scanning Mutagenesis -- Screening Libraries for Improved Solubility: Using E. coli Dihydrofolate Reductase as a Reporter -- In Vitro Directed Evolution of Enzymes Expressed by E. coli in Micro-Titre Plates.
Keywords: Biochemistry; Enzymology; Biochemistry, general; Enzymology