Downstream Processing of Proteins : Methods and Protocols - Desai, Mohamed A.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2000)
Downstream Processing in the Biotechnology Industry -- 2 Microbial Cell Disruption by High-Pressure Homogenization -- 3 Protein Concentration and Buffer Exchange Using Ultrafiltration -- 4 High-Performance Tangential Flow Filtration for Protein Separations -- Large-Scale Recovery of Protein Inclusion Bodies by Continuous Centrifugation -- Application of Density Gradient Ultracentrifugation Using Zonal Rotors in the Large-Scale Purification of Biomolecules -- Application of Chromatography in the Downstream Processing of Biomolecules -- Affinity Ligand Technology -- Immunoaffinity Adsorption in the Large-Scale Isolation of Biomolecules -- Expanded Bed Adsorption in the Purification of Biomolecules -- Application of HPLC in the Purification of Biomolecules -- Countercurrent Chromatography of Proteins with Polymer Phase Systems -- Practical Aspects and Applications of Radial Flow Chromatography -- Sterile Filtration and Virus Filtration -- Design and Interpretation of Viral Clearance Studies for Biopharmaceutical Products -- Validation of Sterilizing Filters in the Biotechnology Industry.
Keywords: Biotechnology; Biotechnology