Dictyostelium discoideum Protocols - Eichinger, Ludwig; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2006)
The Organism, Community Resources, and Genome-wide Studies -- The Secret Lives of Dictyostelium -- The Genome of Dictyostelium discoideum -- The cDNA Sequencing Project -- dictyBase and the Dicty Stock Center -- Analysis of Gene Expression Using cDNA Microarrays -- Proteomic Analysis of Dictyostelium discoideum -- Basic Methods and Molecular Genetic Techniques -- Cultivation, Spore Production, and Mating -- Parasexual Genetics Using Axenic Cells -- Slug Phototaxis, Thermotaxis, and Spontaneous Turning Behavior -- Purification Techniques of Subcellular Compartments for Analytical and Preparative Purposes -- Generation of Multiple Knockout Mutants Using the Cre-loxP System -- Restriction Enzyme-Mediated Integration (REMI) Mutagenesis -- RNA Interference and Antisense-Mediated Gene Silencing in Dictyostelium -- Imaging and Localization Methods -- Application of Fluorescent Protein Tags as Reporters in Live-Cell Imaging Studies -- Investigating Gene Expression -- Application of 2D and 3D DIAS to Motion Analysis of Live Cells in Transmission and Confocal Microscopy Imaging -- Using Quantitative Fluorescence Microscopy and FRET Imaging to Measure Spatiotemporal Signaling Events in Single Living Cells -- Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development -- Under-Agarose Chemotaxis of Dictyostelium discoideum -- Optimized Fixation and Immunofluorescence Staining Methods for Dictyostelium Cells -- Cryofixation Methods for Ultrastructural Studies of Dictyostelium discoideum -- Dictyostelium as Model Organism -- Analysis of Signal Transduction -- Assaying Chemotaxis of Dictyostelium Cells -- Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized Light Scattering -- Quantitative and Microscopic Methods for Studying the Endocytic Pathway -- Preparation of Intact, Highly Purified Phagosomes from Dictyostelium -- Assaying Cell-Cell Adhesion -- Periodic Activation of ERK2 and Partial Involvement of G Protein in ERK2 Activation by cAMP in Dictyostelium Cells -- An Improved Method for Dictyostelium Centrosome Isolation -- Epiggenetics in Dictyostelium -- Dictyostelium discoideum as a Model to Study Host-Pathogen Interactions -- Pharmacogenetics -- How to Assess and Study Cell Death in Dictyostelium discoideum.
Keywords: Biochemistry; Cell biology; Biochemistry, general; Cell Biology