Detection of Blotted Proteins : Methods and Protocols - Kurien, Biji T.; Scofield, R. Hal.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2015)
Western Blotting: Origin and Ascent of the Species -- Methods to Concentrate Proteins for Protein Isolation, Proteomic and Peptidomic Evaluation -- Measuring Protein Concentration on Nitrocellulose and After the Electrophoretic Transfer of Protein to Nitrocellulose -- Detection of Blotted Proteins: Not all Blockers are Created Equal -- Protein Stains to Detect Antigen on Membranes -- Fluorescent Labeling of Proteins and Its Application to SDS-PAGE and Western Blotting -- Rapid, Antibody-free Detection of Recombinant Proteins on Blots Using Enzyme Fragment Complementation -- Use of Non-Radioactive Detection Method for North- and South-Western Blot --  Immunoblotting using Radiolabeled Reagents for Detection -- Immunoblotting of Antigens: Whole, Strip and New-Line Nitrocellulose Membrane Immunoblotting Using the Chemiluminescence Technique -- Detection of Protein Carbonyls by Means of Biotin Hydrazide -Streptavidin Affinity Methods -- Direct Immunodetection of Antigens within the Precast Polyacrylamide Gel -- Quantitative Analysis of Signal Transduction with In-Cell Western Immunofluorescence Assays -- Ultrasensitive Protein Detection on Dot Blots and Western Blots with Semiconducting Polymer Dots -- Co-detection of Target and Total Protein by CyDye Labeling and Fluorescent ECL-plex Immunoblotting in a Standard Proteomics Workflow -- Using Biotinylated Proteins to Demonstrate Immunodetection of Antigens via Western Blotting, Dot blots and Immunohistochemistry -- Calcium Binding by Ro 60 Multiple Antigenic Peptides on PVDF Membrane -- Sequential use of Immunoblots for Characterization of Autoantibody Specificities -- Nanogold Immunodetection Detection Systems for the Identification of Autoantigens by Western Blotting -- Application of Intermittent Microwave Irradiation to Western Blot Analysis -- Visualization of Unstained Protein Bands on PVDF -- Multiplexed Fluorescent Immunodetection Using Low Auto-Fluorescence Immobilon®-FL Membrane -- Cold Microwave-Enabled Protein Detection and Quantification -- TLC-Blot (Far-Eastern Blot) and Its Application to Functional Lipidomics -- Analysis of Electroblotted Proteins by Mass Spectrometry -- On Membrane Renaturation of Recombinant Ro60 Autoantigen by Calcium Ions -- Phosphoprotein Detection on Protein Electroblot Using a Phosphate-Specific Fluorophore -- Purification of Tryptic Digests on Polyvinylidene Difluoride Membrane -- Detection of Blotted Proteins on Nitrocellulose/PVDF Membranes by Alta -- Non-stripping "Rainbow" and Multiple Antigen Detection (MAD) Western Blotting. - Supported Molecular Matrix Electrophoresis -- Parafilm-M®, an Available Cost Effective Alternative for Immuno-blot Pouches -- Succinylation-Alcian Blue Staining of Mucins on Polyvinylidene Difluoride Membranes -- Comparison of Chemiluminescence vs. Infrared Techniques for Detection of Fetuin-A in Saliva -- A Novel Methodology for Stripping and Reprobing of Western Blots Originally Developed with Colorimetric Substrate TMB -- Other Notable Methods of Membrane Protein Detection: A Brief Review -- Nitrocellulose Membrane: The New Canvas -- Invisible Ink Marking in ECL Membrane Assays.    .
Keywords: Proteins .; Protein Science