Glycomics : Methods and Protocols - Packer, Nicolle H.; Karlsson, Niclas G.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Glycan Analysis -- Analysis of N- and O-Linked Glycans from Glycoproteins Using MALDI-TOF Mass Spectrometry -- Infrared Multiphoton Dissociation Mass Spectrometry for Structural Elucidation of Oligosaccharides -- Mass Spectrometry of N-Linked Glycans -- Solid-Phase Permethylation for Glycomic Analysis -- Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization -- Two-Dimensional HPLC Separation with Reverse-Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools After Labeling with 2-Aminobenzamide -- Capillary Lectin-Affinity Electrophoresis for Glycan Analysis -- Analysis of Methylated O-Glycan Alditols by Reversed-Phase NanoLC Coupled CAD-ESI Mass Spectrometry -- High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling -- Collision-Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans -- The Structural Elucidation of Glycosaminoglycans -- Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS Separations -- Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis -- Glycopeptides -- Enrichment Strategies for Glycopeptides -- Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting -- Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF -- N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS -- LC/MS n for Glycoprotein Analysis: N-Linked Glycosylation Analysis and Peptide Sequencing of Glycopeptides -- O-GlcNAcomics -- Detecting the "O-GlcNAcome"; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins -- Glycobioinformatic Tools -- Preparation of a Glycan Library Using a Variety of Glycosyltrasferases -- Data Mining the PDB for Glyco-Related Data -- Carbohydrate-Protein Interactions -- Saccharide Microarrays for High-Throughput Interrogation of Glycan-Protein Binding Interactions -- Glycosaminoglycan Characterization Methodologies: Probing Biomolecular Interactions -- Development and Characterization of Antibodies to Carbohydrate Antigens -- Inhibition of Glycosyltransferase Activities as the Basis for Drug Development -- Saturation Transfer Difference NMR Spectroscopy as a Technique to Investigate Protein-Carbohydrate Interactions in Solution.
Keywords: Biochemistry; Human physiology; Carbohydrates; Biochemistry, general; Human Physiology; Carbohydrate Chemistry