Engineered Zinc Finger Proteins : Methods and Protocols - Mackay, Joel P.; Segal, David J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Engineering Zinc Finger Proteins -- The Generation of Zinc Finger Proteins by Modular Assembly -- Engineering Single Cys2His2 Zinc Finger Domains Using a Bacterial Cell-Based Two-Hybrid Selection System -- Bipartite Selection of Zinc Fingers by Phage Display for Any 9-bp DNA Target Site -- Structure-Based DNA-Binding Prediction and Design -- Artificial Transcription Factors -- Generation of Cell-Permeable Artificial Zinc Finger Protein Variants -- Inhibition of Viral Transcription Using Designed Zinc Finger Proteins -- Modulation of Gene Expression Using Zinc Finger-Based Artificial Transcription Factors -- Construction of Combinatorial Libraries that Encode Zinc Finger-Based Transcription Factors -- Silencing of Gene Expression by Targeted DNA Methylation: Concepts and Approaches -- Remodeling Genomes with Artificial Transcription Factors (ATFs) -- Transgenic Mice Expressing an Artificial Zinc Finger Regulator Targeting an Endogenous Gene -- Zinc Finger Nucleases -- Artificial Zinc Finger Nucleases for DNA Cloning -- In Vitro Assessment of Zinc Finger Nuclease Activity -- Quantification of Zinc Finger Nuclease-Associated Toxicity -- A Rapid and General Assay for Monitoring Endogenous Gene Modification -- Engineered Zinc Finger Proteins for Manipulation of the Human Mitochondrial Genome -- High-Efficiency Gene Targeting in Drosophila with Zinc Finger Nucleases -- Using Zinc Finger Nucleases for Efficient and Heritable Gene Disruption in Zebrafish -- A Transient Assay for Monitoring Zinc Finger Nuclease Activity at Endogenous Plant Gene Targets -- Validation and Expression of Zinc Finger Nucleases in Plant Cells -- Non-FokI-Based Zinc Finger Nucleases -- Beyond Transcription and Cleavage -- Designing and Testing Chimeric Zinc Finger Transposases -- Seeing Genetic and Epigenetic Information Without DNA Denaturation Using Sequence-Enabled Reassembly (SEER) -- Zinc Finger-Mediated Live Cell Imaging in Arabidopsis Roots -- Biophysical Analysis of the Interaction of Toxic Metal Ions and Oxidants with the Zinc Finger Domain of XPA -- Preparation and Zinc-Binding Properties of Multi-Fingered Zinc-Sensing Domains -- Using ChIP-seq Technology to Identify Targets of Zinc Finger Transcription Factors -- Crystallization of Zinc Finger Proteins Bound to DNA -- Beyond DNA: Zinc Finger Domains as RNA-Binding Modules.
Keywords: Proteins .; Genetic engineering; Protein Science; Genetic Engineering