Cystic Fibrosis : Diagnosis and Protocols, Volume II: Methods and Resources to Understand Cystic Fibrosis - Amaral, Margarida D.; Kunzelmann, Karl.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2011)
Introduction to Part I: Overview of Approaches to Study Cystic Fibrosis Pathophysiology -- Imaging CFTR Protein Localization in Cultured Cells and Tissues -- CFTR Regulation of Epithelial Sodium Channel -- Methods for Evaluating Inflammation in Cystic Fibrosis -- Methods for ASL Measurements and Mucus Transport Rates in Cell Cultures -- Measurement of Fluid Secretion from Intact Airway Submucosal Glands -- Measurements of Intracellular Calcium Signals in Polarized Primary Cultures of Normal and Cystic Fibrosis Human Airway Epithelia -- Identification and Quantification of Mucin Expression -- Methods to Classify Bacterial Pathogens in Cystic Fibrosis -- Approaches to Study Differentiation and Repair of Human Airway Epithelial Cells -- Introduction to Part II: Omics in the Biology of Cystic Fibrosis -- Microarray mRNA Expression Profiling to Study Cystic Fibrosis -- Quantitative Differential Proteomics of Cystic Fibrosis Cell Models by SILAC (Stable Isotope Labelling in Cell Culture) -- Application of Mass Spectrometry to Study Proteomics and Interactomics in Cystic Fibrosis -- Functional Genomics Assays to Study CFTR Traffic and ENaC Function -- New Lipidomic Approaches in Cystic Fibrosis -- Introduction to Part III: Resources for CFTR Research -- Primary Epithelial Cell Models for Cystic Fibrosis Research -- Comparative Biology of Cystic Fibrosis Animal Models -- CFTR Folding Consortium: Methods Available for Studies of CFTR Folding and Correction -- Evaluation of the Disease Liability of CFTR Variants.
Keywords: Molecular biology; Human genetics; Laboratory medicine; Molecular Medicine; Human Genetics; Laboratory Medicine