Functional Genomics : Methods and Protocols - Kaufmann, Michael; Klinger, Claudia; Savelsbergh, Andreas; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Predicting RNA Structure with Vfold -- RNA Function Prediction -- Computational Prediction of Novel miRNAs from Genome-Wide Data -- Protein Structure Modeling with MODELLER -- Protein Function Prediction -- Capturing Three-Dimensional Genome Organization in Individual Cells by Single-Cell Hi-C -- Genome-Wide Cell Type-Specific Mapping of In Vivo Chromatin Protein Binding Using an FLP-Inducible DamID System in Drosophila -- DNA Methylation Profiling Using Long-Read Single-Molecule Real-Time Bisulfite Sequencing (SMRT-BS) -- Copy Number Variation Analysis by Droplet Digital PCR -- MicroScale Thermophoresis: A Rapid and Precise Method to Quantify Protein-Nucleic Acid Interactions in Solution -- Establishment of the CRISPR/Cas9 System for Targeted Gene Disruption and Gene Tagging -- Holistic and Affordable Analyses of MicroRNA Expression Profiles Using Tagged cDNA Libraries and a Multiplex Sequencing Strategy -- MicroRNA Expression Analysis Using Small RNA Sequencing Discovery and RT-qPCR-Based Validation -- Using Firefly® Particle Technology for Multiplex MicroRNA Profiling Without RNA Purification -- Multiplex Real-Time PCR Using Encoded Microparticles for MicroRNA Profiling -- Optimized Whole Transcriptome Profiling of Motor Axons -- 2D-DIGE in Proteomics -- STAGE-Diging in Proteomics -- Protein Arrays I: Antibody Arrays -- Protein Arrays II: Antigen Arrays -- Protein Arrays III: Reverse-Phase Protein Arrays -- Isolation of Exosomes for the Purpose of Protein Cargo Analysis with the Use of Mass Spectrometry -- Virus-Induced Gene Silencing (VIGS) and Foreign Gene Expression in Pisum sativum L. Using the 'One-Step' Bean pod mottle virus (BPMV) Viral Vector -- Re-Expressing Epigenetically Silenced Genes by Inducing DNA Demethylation through Targeting of Ten-Eleven Translocation 2 to Any Given Genomic Locus -- Knockdown of Rice MicroRNA166 by Short Tandem Target Mimic (STTM) -- RNAi-Mediated Knockdown of Protein Expression -- Engineered Zinc Finger DNA-Binding Domains: Synthesis, Assessment of DNA-Binding Affinity, and Direct Protein Delivery to Mammalian Cells -- Production, Purification, and Titration of First-Generation Adenovirus Vectors.
Keywords: Human genetics; Human Genetics