Genomics Protocols - Starkey, Mike.; Elaswarapu, Ramnath; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Whole Genome Amplification with Phi29 DNA Polymerase to Enable Genetic or Genomic Analysis of Samples of Low DNA Yield -- Scanning for DNA Variants by Denaturant Capillary Electrophoresis -- Identification of SNPs, or Mutations in Sequence Chromatograms -- BeadArray-Based Genotyping -- Microsatellite-Based Candidate Gene Linkage Analysis Studies -- Full Complexity Genomic Hybridization on 60-mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH) -- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin-Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification -- Application of Microarrays for DNA Methylation Profiling -- Genomewide Identification of Protein Binding Locations Using Chromatin Immunoprecipitation Coupled with Microarray -- Transcriptional Profiling of Small Samples in the Central Nervous System -- Quantitative Expression Profiling of RNA from Formalin-Fixed, Paraffin-Embedded Tissues Using Randomly Assembled Bead Arrays -- Expression Profiling of microRNAs in Cancer Cells: Technical Considerations -- Identification of Disease Biomarkers by Profiling of Serum Proteins Using SELDI-TOF Mass Spectrometry -- The Applicability of a Cluster of Differentiation Monoclonal Antibody Microarray to the Diagnosis of Human Disease -- Protein Profiling Based on Two-Dimensional Difference Gel Electrophoresis -- Quantitative Protein Profiling by Mass Spectrometry Using Isotope-Coded Affinity Tags -- Quantitative Protein Profiling by Mass Spectrometry Using Label-Free Proteomics -- Using 2D-LC-MS/MS to Identify Francisella tularensis Peptides in Extracts from an Infected Mouse Macrophage Cell Line -- Baculovirus Expression Vector System: An Emerging Host for High-Throughput Eukaryotic Protein Expression -- Coimmunoprecipitation and Proteomic Analyses -- Tandem Affinity Purification Combined with Mass Spectrometry to Identify Components of Protein Complexes -- Mammalian Two-Hybrid Assay for Detecting Protein-Protein Interactions in Vivo -- Detection of Protein-Protein Interactions in Live Cells and Animals with Split Firefly Luciferase Protein Fragment Complementation -- Subcellular Localization of Intracellular Human Proteins by Construction of Tagged Fusion Proteins and Transient Expression in COS-7 Cells -- GeneFAS: GeneFAS: A Tool for the Prediction of Gene function Using Multiple Sources of Data -- Comparative Genomics-Based Prediction of Protein Function -- Design, Manufacture, and Assay of the Efficacy of siRNAs for Gene Silencing.
Keywords: Biochemistry; Human genetics; Cell biology; Biotechnology; Biochemistry, general; Human Genetics; Cell Biology; Biotechnology