Gene Knockout Protocols - Kühn, Ralf.; Wurst, Wolfgang; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Gene Modification in ES Cells -- Overview on Mouse Mutagenesis -- Construction of Gene-Targeting Vectors by Recombineering -- Gene-Trap Vectors and Mutagenesis -- Chromosome Engineering in ES Cells -- Gene Modification in Embryonic Stem Cells by Single-Stranded DNA Oligonucleotides -- Generation of shRNA Transgenic Mice -- Mutagenesis of Mouse Embryonic Stem Cells with Ethylmethanesulfonate -- Stem Cell Manipulation -- Gene Targeting in Mouse Embryonic Stem Cells -- Manipulating Mouse Embryonic Stem Cells -- ES Cell Line Establishment -- Generation of Double-Knockout Embryonic Stem Cells -- Differentiation Analysis of Pluripotent Mouse Embryonic Stem (ES) Cells In Vitro -- Cloning of ES Cells and Mice by Nuclear Transfer -- Genetically Engineered Mice -- Isolation, Microinjection and Transfer of Mouse Blastocysts -- Aggregation Chimeras: Combining ES Cells, Diploid, and Tetraploid Embryos -- VelociMouse: Fully ES Cell-Derived F0-Generation Mice Obtained from the Injection of ES Cells into Eight-Cell-Stage Embryos -- Generation of Cre Recombinase-Expressing Transgenic Mice Using Bacterial Artificial Chromosomes -- Inducible Cre Mice -- Creation and Use of a Cre Recombinase Transgenic Database -- Transposon Mutagenesis in Mice -- Lentiviral Transgenesis -- Sperm Cryopreservation and In Vitro Fertilization -- Phenotype Analysis -- Influence of Genetic Background on Genetically Engineered Mouse Phenotypes -- Pathologic Phenotyping of Mutant Mice -- Systemic First-Line Phenotyping.
Keywords: Biochemistry; Human genetics; Animal genetics; Animal models in research; Biochemistry, general; Human Genetics; Animal Genetics and Genomics; Animal Models