Drosophila : Methods and Protocols - Dahmann, Christian; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2008)
Drosophila melanogaster and the Development of Biology in the 20th Century -- Getting Started -- FlyBase -- The Use of P-Element Transposons to Generate Transgenic Flies -- The GAL4 System -- P-Element Mutagenesis -- EMS Screens -- RNAi Screening in Cultured Drosophila Cells -- Methods for Homologous Recombination in Drosophila -- Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis -- Cuticle Preparation of Drosophila Embryos and Larvae -- Immunolabeling of Embryos -- Imaging Cellular and Molecular Dynamics in Live Embryos Using Fluorescent Proteins -- Analysis of Neuromuscular Junctions -- Immunolabeling of Imaginal Discs -- Imaging Drosophila Pupal Wing Morphogenesis -- Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections -- Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues -- Linear RNA Amplification for the Production of Microarray Hybridization Probes -- An Overview of the Identification, Detection, and Functional Analysis of Drosophila MicroRNAs -- Extraction and Immunoblotting of Proteins From Embryos -- Purification of Drosophila Protein Complexes for Mass Spectrometry -- Mass Production of Drosophila Embryos and Chromatographic Purification of Native Protein Complexes -- Flow Cytometric Analysis of Drosophila Cells -- Drosophila Cell Lines as Model Systems and as an Experimental Tool.
Keywords: Entomology; Cell biology; Animal genetics; Entomology; Cell Biology; Animal Genetics and Genomics