Cyclin-Dependent Kinase (CDK) Inhibitors : Methods and Protocols - Orzáez, Mar.; Sancho Medina, Mónica.; Pérez-Payá, Enrique; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Immunoprecipitation of CDK-Cyclin Complexes for Determination of Kinase Activity -- Expression and Purification of Recombinant Cyclins and CDKs for Activity Evaluation -- Expression and Purification of Recombinant CDKs: CDK7, CDK8 and CDK9 -- Preparation of CDK/Cyclin Inhibitor Complexes for Structural Determination -- Fragment-Based De Novo Design of Cyclin-Dependent Kinase 2 Inhibitors --  Protein-Protein Interaction Inspire the De Novo Design of Cyclin-Dependent Kinases Peptide Inhibitors -- Identification of Cyclin A Binders with a Fluorescent Peptide Sensor -- Cell Synchronization Techniques to Study the Action of CDK Inhibitors -- Analysis of CDK Inhibitors Action on Mitochondria-Mediated Apoptosis -- Evaluating the Effects of CDK Inhibitors in Ischemia-Reperfusion Injury Models -- Assessing Cell Cycle Independent Function of the CDK Inhibitor p21CDKN1A in DNA Repair -- Drug Delivery Strategies of Chemical CDK Inhibitors -- Animal Models for Studying the In Vivo Functions of Cell Cycle CDKs -- Evaluating Chemical CDK Inhibitors as Cell Death Inducers -- Models for the Study of the Crosstalk between Inflammation and Cell Cycle -- Metabolomic Applications to the Characterization of the Mode-of-Action of CDK Inhibitors.
Keywords: Pharmacology; Pharmacology/Toxicology.