Glioblastoma : Methods and Protocols - Placantonakis, Dimitris G.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Whole Genome Sequencing-based Discovery of Structural Variants in Glioblastoma -- Whole Genome DNA Methylation Analysis of Human Glioblastoma using Illumina BeadArrays -- Establishing Primary Human Glioblastoma Adherent Cultures from Operative Specimens -- Establishing Primary Human Glioblastoma Tumorsphere Cultures from Operative Specimens -- Isolation of GBM Stem Cells with Flow Cytometry -- Lentiviral Transduction of Primary Human Glioblastoma Cultures -- Selective Targeting of CD133-expressing Glioblastoma Stem Cells using Lentiviral Vectors -- Intracellular pH Measurements in Glioblastoma Cells using the pH-sensitive Dye BCECF -- Induction and Assessment of Hypoxia in Glioblastoma Cells in vitro -- Metabolomic Analysis of Glioma Cells Using Nanoflow Liquid Chromatography-tandem Mass Spectrometry -- Sample Preparation for Relative Quantitation of Proteins Using Tandem Mass Tags (TMT) and Mass Spectrometry (MS) -- Single-Cell RNA Sequencing of Glioblastoma Cells -- Evaluation of Radioresponse and Radiosensitizers in Glioblastoma Organotypic Cultures -- Orthotopic Patient-derived Glioblastoma Xenografts in Mice -- Bioluminescent In Vivo Imaging of Orthotopic Glioblastoma Xenografts in Mice -- Evaluation of Vascularity, Blood Perfusion, and Oxygen Tension in Tumor Xenografts with Fluorescent Microscopy -- Probing Glioblastoma Tissue Heterogeneity with Laser Capture Microdissection -- Flow Cytometric Identification of Tumor-infiltrating Lymphocytes from Glioblastoma -- Modeling Glioma with Human Embryonic Stem Cell-derived Neural Lineages. .
Keywords: Cancer research; Cancer Research