Extracellular Vesicles : Methods and Protocols - Kuo, Winston Patrick; Jia, Shidong; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Extracellular Vesicles: A Brief Overview and Its Role in Precision Medicine -- Red Blood Cells: A Source of Extracellular Vesicles -- Isolation of Extracelullar Vesicles by Ultracentrifugation -- Sequential Filtration: A Gentle Method for the Isolation of Functional Extracellular Vesicles -- Paper-Based Devices for Isolation of Extracellular Vesicles -- Filter-Based Extracellular Vesicle mRNA Isolation and High-Throughput Gene Expression Analysis -- Specific and Generic Isolation of Extracellular Vesicles with Magnetic Beads -- Polymer-Based Purification of Extracellular Vesicles -- Size Exclusion Chromatography: A Simple and Reliable Method for Exosome Purification -- Purification Protocols for Extracellular Vesicles -- Characterization of Extracellular Vesicles by Surface Plasmon Resonance -- Extracellular Vesicle Isolation and Analysis by Western Blotting -- Analysis of Extracellular Vesicles Using Fluorescence Nanoparticle Tracking Analysis -- Characterization of Extracellular Vesicles by Flow Cytometry -- Characterization of Extracellular Vesicles by Size-Exclusion High-Performance Liquid Chromatography (HPLC) -- Multi-Surface Antigen Staining of Larger Extracellular Vesicles -- Microcapillary Chip-Based Extracellular Vesicle Profiling System -- Detection and Characterization of Extracellular Vesicles by Transmission and Cryo-Transmission Electron Microscopy -- Imaging of Isolated Extracellular Vesicles Using Fluorescence Microscopy -- In Vivo Tracking of Extracellular Vesicles in Mice Using Fusion Protein Comprising Lactadherin and Gaussia Luciferase -- Tracking Extracellular Vesicles Delivery and RNA Translation Using Multiplexed Reporters -- Extraction and Analysis of Extracellular Vesicle-Associated miRNAs Following Antibody-Based Extracellular Vesicle Capture from Plasma Samples -- Extracellular Vesicle miRNA Detection Using Molecular Beacons -- Rapid Isolation of Extracellular Vesicles from Blood Plasma with Size-Exclusion Chromatography Followed by Mass Spectrometry-Based Proteomic Profiling -- An Adaptable Polyethylene Glycol-Based Workflow for Proteomic Analysis of Extracellar Vesicles -- Isolation of Extracellular Vesicles from Ovarian Follicular Fluid: Evaluation of Ultracentrifugation and Commercial Kits -- Isolation of Extracellular Vesicles in Saliva Using Density Gradient Ultracentrifugation -- Isolation of Extracellular Vesicles from Breast Milk -- An Integrated Double-Filtration Microfluidic Device for Detection of Extracellular Vesicles from Urine for Bladder Cancer Diagnosis -- Electric Field-Induced Disruption and Releasing Viable Content from Extracellular Vesicles -- Production and Characterization of Extracellular Vesicles in Malaria -- Isolation of Extracellular Vesicles from Stem Cells -- The Use of Peripheral Extracellular Vesicles for Identification of Molecular Biomarkers in a Solid Tumor Mouse Model -- Therapeutic Applications of Extracellular Vesicles: Perspectives from Newborn Medicine -- Therapeutic Use of Tumor Cell-Derived Extracellular Vesicles.
Keywords: Cell biology; Cell Biology