DNA Methylation Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tost, Jörg. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2018.
Edición:3rd ed. 2018.
Colección:Methods in Molecular Biology, 1708
Materias:
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024 7 |a 10.1007/978-1-4939-7481-8  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a DNA Methylation Protocols /  |c edited by Jörg Tost. 
250 |a 3rd ed. 2018. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2018. 
300 |a XVIII, 704 p. 102 illus., 67 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
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490 1 |a Methods in Molecular Biology,  |v 1708 
505 0 |a A Summary of the Biological Processes, Disease-Associated Changes and Clinical Applications of DNA Methylation.- Considerations for Design and Analysis of DNA Methylation Studies.- Quantification of Global DNA Methylation Levels by Mass Spectrometry.- Antibody Based Detection of Global Nuclear DNA Methylation in Cells, Tissue Sections and Mammaliam Embryos.- Whole-Genome Bisulfite Sequencing Using the Ovation® Ultralow Methyl-Seq Protocol.- Tagmentation-Based Library Preparation for Low DNA Input Whole Genome Bisulfite Sequencing.- Post-Bisulfite Adaptor Tagging for PCR-Free Whole-Genome Bisulfite Sequencing.- Multiplexed Reduced Representation Bisulfite Sequencing with Magnetic Bead Fragment Size Selection.- Low Input Whole-Genome Bisulfite Sequencing Using a Post-Bisulfite Adapter Tagging Approach.- Methyl-CpG Binding Domain Sequencing: MBD-seq.- The HELP-Based Assays.- Comprehensive Whole DNA Methylome Analysis by InDigital Restriction Enzyme Analysis of Methylation (DREAM).- Nucleosome Occupancy and Methylome Sequencing (NOMe-seq).- Bisulfite Sequencing of Chromatin Immunoprecipitated DNA (BisChIP-seq).- A Guide to Illumina BeadChip Data Analysis.- Microdroplet PCR for Highly-Multiplexed Targeted Bisulfite Sequencing.- Multiplexed DNA Methylation Analysis of Target Regions Using Microfluidics (Fluidigm) -- Large-Scale Targeted DNA Methylation Analysis Using Bisulfite Padlock Probes.- Targeted Bisulfite Sequencing Using the SeqCap Epi Enrichment System.- Multiplexed and Sensitive DNA Methylation Testing Using Methylation-Sensitive Restriction Enzymes MSRE-qPCR.- Quantitative DNA Methylation Analysis at Single-Nucleotide Resolution by Pyrosequencing®.- Methylation-Specific PCR.- Quantitation of DNA Methylation by Quantitative Multiplex Methylation-Specific PCR (QM-MSP) -- MethyLight and Digital MethyLight.- Quantitative Region-Specific DNA Methylation Analysis by the EPITYPERTM Technology.- Methylation-Specific Multiplex Ligation-Dependent Probe Amplification  (MS-MLPA).- Methylation - Sensitive - High Resolution Melting (MS-HRM).- Hairpin Bisulfite Sequencing: Synchronous Methylation Analysis on Complementary DNA Strands of Individual Chromosomes.- Helper-Dependent Chain Reaction (HDCR) for Selective Amplification of Methylated DNA Sequences.- DNA Methylation Analysis from Blood Spots: Increasing Yield and Quality for Genome-Wide and Locus-Specific Methylation.- DNA Methylation Analysis of Free-Circulating DNA in Body Fluids.- Tet-Assisted Bisulfite Sequencing (TAB-seq).- Multiplexing for Oxidative Bisulfite Sequencing (oxBS-seq).- Affinity-Based Enrichment Techniques for the Genome-Wide Analysis of 5-Hydroxymethylcytosine. 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Tost, Jörg.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)