Extracellular Matrix Protocols : Second Edition - Even-Ram, Sharona; Artym, Vira.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Molecular Biology -- Retroviral delivery of ECM genes -- Tissue-Specific KO of ECM Proteins -- Recombinant Collagen Trimers from Insect Cells and Yeast -- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag -- Preparation of recombinant fibronectin fragments for functional and structural studies -- Quantitative Determination of Collagen Cross-links -- Biochemical and biophysical analysis -- ECM Macromolecules: Height-mapping and Nano-mechanics Using Atomic Force Microscopy -- Atomic Force Microscopy Measurements of Intermolecular Binding Forces -- Mass-Mapping of ECM Macromolecules by Scanning Transmission Electron Microscopy -- Chemical Microscopy of Biological Samples by Dynamic Mode Secondary Ion Mass Spectrometry (SIMS) -- ECM Macromolecules: Rotary Shadowing and Transmission Electron Microscopy -- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Substrates -- Solid Phase Assays for Studying ECM Protein-Protein Interactions -- Cell Adhesion Assays -- Cell biology assays -- ECM Degradation Assays for Analyzing Local Cell Invasion -- Fluorescence-Based Assays for In Vitro Analysis of Cell Adhesion and Migration -- Fibrin Gel Model for Assessment of Cellular Contractility -- Fluorescent Labeling Techniques for Investigation of Fibronectin Fibrillogenesis (Labeling Fibronectin Fibrillogenesis) -- Stromagenesis During Tumorigenesis: Characterization of Tumor-associated Fibroblasts and Stroma-derived 3D Matrices -- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes -- Organ models -- ECM and FGF-Dependent Assay of Embryonic SMG Epithelial Morphogenesis: Investigating Growth Factor/Matrix Regulation of Gene Expression During Submandibular Gland Development -- Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary Tissue from Knockout Mice -- Tissue Engineering -- Characterizing ECM Production by Cells Encapsulated in Hydrogels -- Tissue Engineering and Cell-Populated Collagen Matrices.
Keywords: Human anatomy; Cell biology; Cell membranes .; Anatomy; Cell Biology; Membrane Biology