Germinal Centers : Methods and Protocols - Calado, Dinis Pedro; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Analysis of the Germinal Center Reaction in Tissue Sections -- Detecting Gene Expression in Lymphoid Microenvironments by Laser Microdissection and Quantitative RT-PCR -- Tracking Plasma Cell Differentiation in Living Mice with 2-Photon Microscopy -- Microanatomical Labeling of Germinal Center Structures for Flow Cytometry Using Photoactivation -- Fate Mapping and Transcript Profiling of Germinal Center Cells by Two-photon Photoconversion -- Intravital Microscopy of T-B Cell Interactions in Germinal Centers -- Identifying Follicular Regulatory T cells by Confocal Microscopy -- Cytokine Expression by T follicular Helper Cells -- Follicular Dendritic Cell Isolation and Loading of Immune Complexes -- Isolation and Characterization of Mouse and Human Follicular Dendritic Cells -- In-vitro-induced Germinal Center B Cell Culture System -- CRISPR/Cas9 Mediated In Vitro Mutagenesis in GC-like B Cells -- Germinal Center Formation with Retrovirally-transduced B Cells for Determining the Role of Specific Molecules In Vivo -- Characterization of the B Cell Transcriptome by RNA-Binding Proteins using iCLIP -- Flow-Cytometric Method Measuring B Cell Surface Immunoglobulin Avidity -- Somatic Hypermutation and Affinity Maturation Analysis Using the 4-hydroxy-3-nitrophenyl-acetyl (NP) System -- Targeting Gene Function in Germinal Center B Cells: A Practical Approach -- Development of Mouse Model Systems of Germinal Centre Lymphomas -- The AID-Cre-ERT2 Model: A Tool for Monitoring B Cell Immune Responses and Generating Selective Hybridomas -- Determining the Origin of Human Germinal Center B Cell-derived Malignancies -- Tracking B-cell Repertoires and Clonal Histories in Normal and Malignant Lymphocytes -- How to Simulate a Germinal Centre. .
Keywords: Immunology; Immunology