DNA'Protein Interactions : Principles and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Moss, Tom. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2001.
Edición:2nd ed. 2001.
Colección:Methods in Molecular Biology, 148
Materias:
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040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a DNA'Protein Interactions :  |b Principles and Protocols /  |c edited by Tom Moss. 
250 |a 2nd ed. 2001. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2001. 
300 |a 656 p. 146 illus. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 148 
505 0 |a Filter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Uranyl Photofootprinting -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ultraviolet-Laser Footprinting -- In Vivo DNA Analysis -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Ethylation Interference -- Hydroxyl Radical Interference -- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Chemical Modification of Lysine by Reductive Methylation -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Site-Specific Protein-DNA Photocrosslinking -- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex -- UV Laser-Induced Protein-DNA Crosslinking -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Engineering Nucleic Acid-Binding Proteins by Phage Display -- Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast -- Assays for Transcription Factor Activity -- Assay of Restriction Endonucleases Using Oligonucleotides -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Calorimetry of Protein-DNA Complexes and Their Components -- Surface Plasmon Resonance Applied to DNA-Protein Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Two-Dimensional Crystallization of Soluble Protein Complexes -- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Scanning Transmission Electron Microscopy of DNA-Protein Complexes -- Determination of Nucleic Acid Recognition Sequences by SELEX -- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation. 
650 0 |a Cell biology. 
650 1 4 |a Cell Biology. 
700 1 |a Moss, Tom.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks