DNA'Protein Interactions : Principles and Protocols - Moss, Tom.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2001)
Filter-Binding Assays -- Electrophoretic Mobility Shift Assays for the Analysis of DNA-Protein Interactions -- DNase I Footprinting -- Footprinting with Exonuclease III -- Hydroxyl Radical Footprinting -- The Use of Diethyl Pyrocarbonate and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA -- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper -- Uranyl Photofootprinting -- Osmium Tetroxide Modification and the Study of DNA-Protein Interactions -- Determination of a Transcription-Factor-Binding Site by Nuclease Protection Footprinting onto Southwestern Blots -- Diffusible Singlet Oxygen as a Probe of DNA Deformation -- Ultraviolet-Laser Footprinting -- In Vivo DNA Analysis -- Identification of Protein-DNA Contacts with Dimethyl Sulfate -- Ethylation Interference -- Hydroxyl Radical Interference -- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting -- A Competition Assay for DNA Binding Using the Fluorescent Probe ANS -- Site-Directed Cleavage of DNA by Linker Histone-Fe(II) EDTA Conjugates -- Nitration of Tyrosine Residues in Protein-Nucleic Acid Complexes -- Chemical Modification of Lysine by Reductive Methylation -- Limited Proteolysis of Protein-Nucleic Acid Complexes -- Ultraviolet Crosslinking of DNA-Protein Complexes via 8-Azidoadenine -- Site-Specific Protein-DNA Photocrosslinking -- Site-Directed DNA Photoaffinity Labeling of RNA Polymerase III Transcription Complexes -- Use of Site-Specific Protein-DNA Photocrosslinking to Analyze the Molecular Organization of the RNA Polymerase II Initiation Complex -- UV Laser-Induced Protein-DNA Crosslinking -- Plasmid Vectors for the Analysis of Protein-Induced DNA Bending -- Engineering Nucleic Acid-Binding Proteins by Phage Display -- Genetic Analysis of DNA-Protein Interactions Using a Reporter Gene Assay in Yeast -- Assays for Transcription Factor Activity -- Assay of Restriction Endonucleases Using Oligonucleotides -- Analysis of DNA-Protein Interactions by Intrinsic Fluorescence -- Circular Dichroism for the Analysis of Protein-DNA Interactions -- Calorimetry of Protein-DNA Complexes and Their Components -- Surface Plasmon Resonance Applied to DNA-Protein Complexes -- Reconstitution of Protein-DNA Complexes for Crystallization -- Two-Dimensional Crystallization of Soluble Protein Complexes -- Atomic Force Microscopy of DNA and Protein-DNA Complexes Using Functionalized Mica Substrates -- Electron Microscopy of Protein-Nucleic Acid Complexes -- Scanning Transmission Electron Microscopy of DNA-Protein Complexes -- Determination of Nucleic Acid Recognition Sequences by SELEX -- High DNA-Protein Crosslinking Yield with Two-Wavelength Femtosecond Laser Irradiation.
Keywords: Cell biology; Cell Biology