DNA Topoisomerases : Methods and Protocols - Clarke, Duncan J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
1. Introduction: Emerging Themes in DNA Topoisomerase Research -- 2. Analysis of DNA Topoisomers, Knots, and Catenanes by Agarose Gel Electrophoresis -- 3. Two-Dimensional Agarose Gel Electrophoresis of DNA Topoisomers -- 4. Cleavage of Plasmid DNA by Eukaryotic Topoisomerase II -- 5. Assays for the Preferential Binding of Human Topoisomerase I to Supercoiled DNA -- 6. Monitoring the Topoisomerase II DNA Gate Conformational Change with Fluorescence Resonance Energy Transfer -- 7. Single-Molecule Magnetic Tweezers Studies of Type IB Topoisomerases -- 8. Dissolution of Double Holliday Junctions by the Concerted Action of BLM and Topoisomerase III? -- 9. ChIP-on-Chip Analysis of DNA Topoisomerases -- 10. Analyzing Top2 Distribution on Yeast Chromosomes by Chromatin Immunoprecipitation -- 11. Binding of DNA Topoisomerases I and II to Replication Origins -- 12. Cytometric Assessment of DNA Damage Induced by DNA Topoisomerase Inhibitors -- 13. Analysis of the Topoisomerase II-Dependent Decatenation G2 Checkpoint and Checkpoint Kinases in Human Cells -- 14. Assaying Topoisomerase II Checkpoints in Yeast -- 15. Cytological Analysis of Chromosome Structural Defects that Result from Topoisomerase II Dysfunction -- 16. Top2 SUMO Conjugation in Yeast Cell Lysates -- 17. Analysis of SUMOylation of Topoisomerase II? with Xenopus Egg Extracts -- 18. The Dynamics of DNA Topoisomerase II? in Living Cells -- 19. Depletion and Mutation of Topoisomerase II in Animal Cells.
Keywords: Human genetics; Biochemistry; Enzymology; Human Genetics; Biochemistry, general; Enzymology