High-Throughput Protein Production and Purification : Methods and Protocols - Vincentelli, Renaud; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Part 1: General procedures for high-throughput protein production -- Overview of high-throughput cloning methods for the post-genomic era -- Overview of a high-throughput pipeline for streamlining the production of recombinant proteins -- Semi-automated small-scale purification method for high-throughput expression analysis of recombinant proteins -- High-Throughput Protein Production in Yeast -- A High-Throughput System for Transient and Stable Protein Production in Mammalian Cells -- A High-throughput Automated Protein Folding System -- Part 2: High-throughput Protocols adapted to the production of specific protein families -- High-throughput production of oxidized animal toxins in Escherichia coli -- High-Throughput purification of protein kinases in Escherichia coli and insect cells -- Parallelized micro-scale expression of soluble scFv -- High throughput production of influenza virus-like particle (VLP) array by using VLP-factoryTM, a MultiBac baculoviral genome customized for enveloped VLP expression -- High-throughput Protein Production of membrane proteins in Saccharomyces cerevisiae -- High-throughput E. coli cell-free expression: From PCR product design to functional validation of GPCR -- High-Throughput Site-Directed Mutagenesis -- Part 3: High-throughput protein production combined with High-throughput micro-characterization for protein stability, solubility, quality and interactions -- Hot CoFi blot - A High-throughput colony-based screen for identifying more thermally stable protein variants -- High-throughput isolation of soluble protein domains using the bipartite split-GFP system -- High-throughput Analytical Light Scattering for Protein Quality Control and Characterization -- High-throughput Nano-Scale Characterization of Membrane Proteins Using Fluorescence-Detection Size-Exclusion Chromatography -- A Flexible and Scalable High-Throughput Platform for Recombinant Membrane Protein Production -- Adaption of the Leishmania cell-free expression system to high-throughput analysis of protein interactions -- High-throughput protein-protein interaction assays using tripartite split-GFP complementation -- High-throughput production of a new library of human single and tandem PDZ domains allows quantitative PDZ-peptide interaction screening through high throughput holdup assay -- High throughput protein analysis using Negative Stain Electron Microscopy and 2D classification -- High-throughput protein production combined with High throughput SELEX identifies an extensive atlas of Ciona robusta transcription factor DNA-binding specificities -- High-throughput Micro-characterization of RNA-protein interactions.
Keywords: Proteins .; Protein Science