Microbial Metabolic Engineering : Methods and Protocols - Santos, Christine Nicole S.; Ajikumar, Parayil Kumaran; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Pathway and Gene Discovery from Natural Hosts and Organisms -- Metabolic In silico Network Expansions to Predict and Exploit Enzyme Promiscuity -- Antisense RNA Elements for Downregulating Expression -- Chemically Inducible Chromosomal Evolution (CIChE) for Multi-copy Metabolic Pathway Engineering -- Bacterial Expression of Membrane Associated Cytochrome P450s and their Activity Assay in Nanodiscs -- Delta Integration CRISPR-Cas (Di-CRISPR) in Saccharomyces cerevisiae -- Assembly of Complex Pathways using Type IIs Restriction Enzymes -- Combinatorial Assembly of Multigene Pathways by Combining Single Strand Assembly with Golden Gate Assembly -- Checks and Balances with Use of the Keio Collection for Phenotype Testing -- Genetic Engineering of Cyanobacteria: Design, Implementation, and Characterization of Recombinant Synechocystis sp. PCC 6803 -- Genetic Tools for Streamlined and Accelerated Pathway Engineering in Yarrowia lipolytica -- Gas Chromatography-mass Spectrometry Microbial Metabolomics for Applications in Strain Optimization -- Targeted Mass Spectrometry-based Proteomics Tools for Strain Optimization -- A Protocol for the Construction and Curation of Genome-scale Integrated Metabolic and Regulatory Network Models -- 13C-Fingerprinting and Metabolic Flux Analysis of Microbial Metabolisms -- A Genetic Screen for the Isolation of Mutants with Increased Flux in the Isoprenoid Pathway of Yeast.
Keywords: Biomedical engineering; Microbiology; Biomedical Engineering/Biotechnology.; Microbiology