Mass Cytometry : Methods and Protocols - McGuire, Helen M.; Ashhurst, Thomas M.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Setting up Mass Cytometry in a Shared Resource Lab Environment -- Acquisition, Processing, and Quality Control of Mass Cytometry Data -- Visualization of Mass Cytometry Signal Background to Enable Optimal Core Panel Customization and Signal Threshold Gating -- Method for Tagging Antibodies with Metals for Mass Cytometry Experiments -- Scalable Conjugation and Characterization of Immunoglobulins with Stable Mass Isotope Reporters for Single-Cell Mass Cytometry Analysis -- Titration of Mass Cytometry Reagents -- Cell-Surface Barcoding of Live PBMC for Multiplexed Mass Cytometry -- Automated Cell Processing for Mass Cytometry Experiments -- Live Cell Barcoding for Efficient Analysis of Small Samples by Mass Cytometry -- Staining of Phosphorylated Signalling Markers Protocol for Mass Cytometry -- Multiplex MHC Class I Tetramer Combined with Intranuclear Staining by Mass Cytometry -- Analysis of the Murine Bone Marrow Haematopoietic System Using Mass and Flow Cytometry -- Mass Cytometric Cell Cycle Analysis -- Picturing Polarized Myeloid Phagocytes and Regulatory Cells by Mass Cytometry -- Quantitative Measurement of Cell-nanoparticle Interactions Using Mass Cytometry -- Data-Driven Flow Cytometry Analysis -- Analysis of Mass Cytometry Data -- Analysis of High Dimensional Phenotype Data Generated by Mass Cytometry or High Dimensional Flow Cytometry -- Computational Analysis of High Dimensional Mass Cytometry Data from Clinical Tissue Samples -- Supervised Machine Learning with CITRUS for Single Cell Biomarker Discovery.
Keywords: Cell biology; Mass spectrometry; Antibodies; Cell Biology; Mass Spectrometry; Antibodies