Insect Cell Cultures : Fundamental and Applied Aspects - Vlak, Just M.; de Gooijer, Cornelis D.; Tramper, Johannes; Miltenburger, Herbert G.; SpringerLink (Online service) (Dordrecht : Springer Netherlands : Imprint: Springer, 2002)
Insect Cell Lines -- Development and characterization of insect cell lines -- New approaches to insect tissue culture -- Transgenic insect cells: mosquito cell mutants and the dihydrofolate reductase gene -- Insect cell physiology -- Insect cell cultivation: growth and kinetics -- Medium design for insect cell culture -- Viruses -- Baculovirus-insect cell interactions -- Replication patterns and cytopathology of cells infected with baculoviruses -- Construction of baculovirus recombinants -- Passage effect of virus infection in insect cells -- Post-translational modifications in insect cells -- Productivity of insect cells for recombinant proteins -- Chaperone and foldase coexpression in the baculovirus-insect cell expression system -- Engineering Aspects -- Shear sensitivity of insect cells -- Insect cell bioreactors -- Perfusion bioreactors for the production of recombinant proteins in insect cells -- Immobilization of insect cells -- Modelling baculovirus infection of insect cells in culture -- Scale up aspects of sparged insect-cell bioreactors -- Oxygen gradients in small and big sparged insect-cell bioreactors -- Downstream processing of insect cell cultures -- Applications -- Parvovirus diagnostics and vaccine production in insect cells -- Classical swine fever virus diagnostics and vaccine production in insect cells -- Production of multidomain complement glycoproteins in insect cells -- Economic and Regulatory Aspects -- Economics of baculovirus-insect cell production systems -- Safety aspects of insect cell culture -- Regulatory issues in the use of insect-cell culture.
Keywords: Entomology; Animal anatomy; Biochemistry; Biotechnology; Entomology; Animal Anatomy / Morphology / Histology; Animal Biochemistry; Biotechnology