Inositol Phosphates and Lipids : Methods and Protocols - Barker, Christopher J.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
The Role of Inositol and the Principles of Labelling, Extraction, and Analysis of Inositides in Mammalian Cells -- HPLC Separation of Inositol Polyphosphates -- Molecular Manipulation and Analysis of Inositol Phosphate and Pyrophosphate Levels in Mammalian Cells -- A Femtomole-Sensitivity Mass Assay for Inositol Hexakisphosphate -- Synthesis of InsP 7 by the Inositol Hexakisphosphate Kinase 1 (IP6K1) -- Protein Pyrophosphorylation by Diphosphoinositol Pentakisphosphate (InsP 7) -- Synthesis and Nonradioactive Micro-analysis of Diphosphoinositol Phosphates by HPLC with Postcolumn Complexometry -- Diphosphosinositol Polyphosphates and Energy Metabolism: Assay for ATP/ADP Ratio -- Isolation of Inositol 1,4,5-Trisphosphate Receptor-Associating Proteins and Selective Knockdown Using RNA Interference -- Phosphoinositide-Specific Phospholipase C ?1 Signal Transduction in the Nucleus -- Methods to Assess Changes in the Pattern of Nuclear Phosphoinositides -- Measurement of Phosphoinositide 3-Kinase Products in Cultured Mammalian Cells by HPLC -- Phosphoinositide Analysis by Liquid Chromatography-Mass Spectrometry -- Imaging Phosphoinositide Dynamics in Living Cells.
Keywords: Cell biology; Cell physiology; Cell Biology; Cell Physiology