Imaging Gene Expression : Methods and Protocols - Shav-Tal, Yaron; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Imaging Single mRNA Molecules in Mammalian Cells using an Optimized MS2-MCP System -- Live-Cell Imaging of Long Non-Coding RNAs using Molecular Beacons -- Real-Time Observation of Localization and EXpression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene -- Visualization of Single mRNAs in Live Neurons -- Lightening Up Gene Activation in Living Drosophila Embryos -- Quantification of mRNA Turnover in Living Cells: A Pipeline for TREAT Data Analysis -- Imaging rRNA Methylation in Bacteria by MR-FISH -- Detection of mRNA Transfer between Mammalian Cells in Co-Culture by Single Molecule Fluorescent In Situ Hybridization (smFISH) -- Live-Cell Imaging of mRNP-NPC Interactions in Budding Yeast -- Detection of mRNAs Anchored to the Nuclear Envelope during Export Inhibition in Living Cells -- Programmable Chromosome Painting with Oligopaints -- Imaging Chromosome Territory and Gene Loci Positions in Cells Grown on Soft Matrices -- Targeted Manipulation/Repositioning of Subcellular Structures and Molecules -- Visualization of Endogenous Transcription Factors in Single Cells using an Antibody Electroporation-Based Imaging Approach -- Monitoring Transcription Factor Oligomerization in Single Living Cells by Numbers and Brightness Analysis -- Visualizing Transcription Factor Binding on Mitotic Chromosomes using Single Molecule Live-Cell Imaging -- Light-Induced Transcription Activation for Time-Lapse Microscopy Experiments in Living Cells.
Keywords: Gene expression; Microscopy; Gene Expression; Biological Microscopy