Leishmania : Methods and Protocols - Clos, Joachim; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Host-Free Systems for Differentiation of Axenic Leishmania -- Phylogenetic Studies -- A Guide to Next Generation Sequence Analysis of Leishmania Genomes -- Quantitative RNA Analysis using RNA Seq -- Ribosome Profiling in Trypanosomatids -- Cosmid Library Construction and Functional Cloning -- Cos-Seq, A High Throughput Gain of Function Screen for Drug Resistance Studies in Leishmania -- Gene Replacement by Homologous Recombination -- LeishGEdit: A Method for Rapid Gene Knockout and Tagging using CRISPR-Cas9 -- DiCre-Based Inducible Disruption of Leishmania Genes -- DiCre-Based Inducible Gene Expression -- Generation of Bone Marrow-Derived Macrophages for In Vitro Infection Experiments -- Quantification of Intracellular Leishmania spp. Using Real-Time Quantitative PCR (qPCR) -- In Vitro Infections of Macrophage-Like Cell Lines with Leishmania infantum for Drug Screening -- Quantification of Parasite Loads by Automated Microscopic Image Analysis -- Quantification of Leishmania Parasites in Murine Models of Visceral Infection -- Syrian Hamster as an Advanced Experimental Model for Visceral Leishmaniasis -- Experimental Cutaneous Leishmaniasis: Mouse Models for Resolution of Inflammation versus Chronicity of Disease -- Establishment, Maintenance of Phlemotomus spp. In the Laboratory, and Infection with Leishmania spp.
Keywords: Infectious diseases; Infectious Diseases