Lysosomes : Methods and Protocols - Öllinger, Karin; Appelqvist, Hanna; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS -- Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins -- Analyzing Lysosome Related Organelles By Electron Microscopy -- Microscopic Analysis of Lysosomal Membrane Permeabilization -- Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets -- Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues -- Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery -- Delivery of Cargo to Lysosomes using Gneosomes -- Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles -- Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells -- Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry -- Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes -- Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates -- Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex -- Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms -- Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples -- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi -- Lysosomal Biology in Cancer. .
Keywords: Cell biology; Cell Biology