Lipases and Phospholipases : Methods and Protocols - Sandoval, Georgina; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2018)
Part 1. Overview of Lipases, Phospholipases and Esterases -- Lipases: An Overview -- Carbohydrate Esterases: An Overview -- Phospholipases: An Overview -- Part 2. Activity Screening and Expression -- Functional-Based Screening Methods for Detecting Esterase and Lipase Activity Against Multiple Substrates -- A Continuous and Sensitive Spectrophotometric Assay for Lipase and Phospholipase A Activities using α-Eleostearic Acid-Containing Substrates -- Direct and Continuous Measurement of Phospholipase D Activities Using the Chelation-Enhanced Fluorescence Property of 8-Hydroxyquinoline -- Lipase and Phospholipase Activity Methods for Marine Organisms -- Plant Lipases: Functional Expression of CPLIP1 from Carica papaya -- Heterologous Expression and Functional Characterization of Sparidae Fish Digestive Phospholipase A2 -- Expression and Purification of Recombinant Vigna unguiculata Phospholipase D in Pichia pastoris for Structural Studies -- Part 3. Optimization of the Biocatalyst Production and Performances -- Recent Advances in Pichia pastoris as Host for Heterologous Expression Systems for Lipases (Review) -- Solid-State Fermentation as an Economic Production Method of Lipases and Esterases -- Synthetic Biology to Improve the Production of Lipases and Esterases (Review) -- Rational Design Strategy as a Novel Immobilization Methodology Applied to Lipases and Phospholipases -- Part 4. Applications of Lipases, Phospholipases and Esterases -- Lipase-Catalyzed Synthesis of Fatty Acid Esters of Trisaccharides -- Targeting Phospholipase D genetically and Pharmacologically for Studying Leukocyte Function -- Lipase-Catalyzed Synthesis of Structured Lipids -- Lipase-Catalyzed Acetylation and Esterification of Bile Acids -- Lipases in Green Chemistry: Deep Eutectic Solvents (DES) as New Green Solvents -- Chemoenzymatic Synthesis of Nitrogen Polymers with Biomedical Applications Catalyzed by Lipases -- Lipases and Esterases as Biocatalyst for Biodiesel Production (Review) -- Lipases, Phospholipases and Esterases Biosensors (Review).
Keywords: Biochemistry; Biochemistry, general