Human Monoclonal Antibodies : Methods and Protocols - Steinitz, Michael; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Human Monocolonal Antibodies: The Benefits of Humanization -- Cancer Immunotherapy: The Dawn of Antibody Cocktails -- IgM Natural Autoantibodies in Physiology and the Treatment of Disease -- Monoclonal Antibodies for the Treatment of Melanoma: Present and Future Strategies -- An Efficient Method to Generate Monoclonal Antibodies from Human B Cells -- Isolation of Antigen-Specific, antibody-Secreting Cells using a Chip-Based Immunospot Array -- Purification of Human Monoclonal antibodies and Their Fragments -- One-Tube Multicolor Flow Cytometry Assay (OTMA) for Comprehensive Immunophenotyping of Peripheral Blood -- Humanization and Simultaneous Optimization of Monoclonal Antibody -- Antibody Fragments Humanization: Beginning with the End in Mind -- Antigen-Specific Human Monoclonal Antibodies from Transgenic Mice -- Refining the Quality of Monoclonal Antibodies: Grafting Unique Peptide-Binding Site in the Fab Framework -- Basic Procedures for Detection and Cytotoxicity of Chimeric Antigen Receptors -- Rapid Chimerization of Antibodies -- Phage Display Technology for Human Monoclonal Antibodies -- Recombinant Antibody Selections by Combining Phage and Yeast Display -- Epitope Mapping via Phage Display from Single Gene Libraries -- A High-Throughput Magnetic Nanoparticle Based Semi-Automated Antibody Phage Display Biopanning -- A Binding Potency Assay for Pritumumab and Ecto-Domain Vimentin -- A Method to Detect the Binding of Hyper-Glycosylated Fragment Crystallizable (Fc) Region of Human IgG1 to Glycan Receptors -- A Cell-Based Reporter Assay Measuring the Activation of Fc Gamma Receptors Induced by Therapeutic Monoclonal Antibodies -- "BIClonals": Production of Bispecific Antibodies in IgG Format in Transiently Transfected Mammalian Cells -- Production of Stabilized Antibody Fragments in the E. coli Bacterial Cytoplasm and in Transiently-Transfected Mammalian Cells.
Keywords: Antibodies; Antibodies