Hepatitis C Virus Protocols - Law, Mansun; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Overview of Direct Acting Antiviral Drugs and Drug Resistance of Hepatitis C Virus -- Similarities and Differences between HCV Pseudoparticle (HCVpp) and Cell Culture HCV (HCVcc) in the Study of HCV -- Hepatitis C Virus Database and Bioinformatics Analysis Tools in the Virus Pathogen Resource (ViPR) -- Establishment of Replication-competent HCV Strain with Minimum Modifications -- Full-length Open Reading Frame Amplification of Hepatitis C Virus -- InFusion Cloning for the Generation of Biologically Relevant HCV Chimeric Molecular Clones -- Production and Purification of Cell Culture Hepatitis C Virus -- Embryonic or Induced Pluripotent Stem Cell-derived Hepatocellular Systems for HCV Culture -- Molecular Identification of Transmitted/Founder Hepatitis C Viruses and their Progeny by Single Genome Sequencing -- A Cell Culture Model for Persistent HCV Infection -- Expression Cloning of Host Factors Required for the HCV Replication Cycle -- Application of Deep Mutational Scanning in Hepatitis C Virus -- Studying the Hepatitis C Virus-induced Epigenetic Signature after Cure with Direct-acting Antivirals -- Measuring Hepatitis C Virus Envelopment by using a Proteinase K Protection Assay -- Membrane Fusion Assays for Studying Entry Hepatitis C Virus into Cells -- Functional Characterization of Apolipoproteins in the HCV Life Cycle -- Live Cell Imaging of Hepatitis C Virus Trafficking in Hepatocytes -- Cloning and Analysis of Authentic Patient-derived HCV E1/E2 Glycoproteins -- Simultaneous Quantification of Hepatitis C virus Envelope Glycoproteins E1 and E2 by Dual-color Fluorescence Immunoblot Analysis -- Overcoming Challenges of Hepatitis C Virus Envelope Glycoprotein Production in Mammalian Cells -- Monitoring of Interferon Response Triggered by Cells Infected by Hepatitis C Virus or Other Viruses upon Cell-cell Contact -- Complement Regulation and Immune Evasion by Hepatitis C Virus -- Direct ex vivo Functional Analysis of HCV-specific T Cells -- Simultaneous Analysis of HCV-specific CD4+ and CD8+ T Cells by Multi-color Flow Cytometry -- Probing the Antigenicity of HCV Envelope Glycoproteins by Phage Display Antibody Technology. -Isolation of HCV Neutralizing Antibodies by Yeast Display -- Detection of Antibodies to HCV E1E2 by Lectin-Capture ELISA -- In vitro Neutralization Assay using Cultured Hepatitis C Virus -- Standardized Method for the Study of Antibody Neutralization of HCV pseudoparticles (HCVpp) -- Detection of Antigen-specific CD8+ T Cells in the Livers of HCV Core Transgenic Mice -- Mice with Chimeric Human Livers and Their Applications -- Mouse Models for Studying HCV Vaccines and Therapeutic Antibodies.
Keywords: Immunology; Virology; Immunology; Virology