Lipid-Activated Nuclear Receptors : Methods and Protocols - Gage, Matthew C.; Pineda-Torra, Inés.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2019)
Quantification of Oxysterol Nuclear Receptor Ligands by LC/MS/MS -- A Stable Luciferase Reporter System to Characterise LXR Regulation by Oxysterols and Novel Ligands -- Isolation and Analysis of Human Monocytes and Adipose Tissue Macrophages -- Glucan Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Adipose Tissue Macrophages -- Isolation and Purification of Tissue Resident Macrophages for the Analysis of Nuclear Receptor Activity -- Bone Marrow-Derived Macrophage Immortalization of LXR Nuclear Receptor-Deficient Cells -- Dual Crosslinking Chromatin Immunoprecipitation Protocol for Next Generation Sequencing (ChIPseq) in Macrophages -- Analysis of LXR Nuclear Receptor Cistrome through ChIP-seq Data Bioinformatics -- Quantifying Cellular Cholesterol Efflux -- Methods for Assessing the Effects of LXR Agonists on Macrophage Bacterial Infection -- Measuring Apoptotic Cell Engulfment (Efferocytosis) Efficiency -- Methods to Study Monocyte and Macrophage Trafficking in Atherosclerosis Progression and Resolution -- Preparation of Frozen Liver Tissues for Integrated Omics Analysis -- Identification of Nuclear Receptor Targets By Chromatin Immunoprecipitation in Fatty Liver -- The LPS/D-Galactosamine-Induced Fulminant Hepatitis Model to Assess the Role of Ligand-Activated Nuclear Receptors on the NLRP3 Inflammasome Pathway In Vivo -- Analyzing T-Cell Plasma Membrane Lipids by Flow Cytometry.
Keywords: Proteins .; Lipids; Receptors; Lipidology