Hypertension : Methods and Protocols - Touyz, Rhian M.; Schiffrin, Ernesto L.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Large Scale Transcriptome Analysis -- Methods to Assess Genetic Risk Prediction -- Microarray Analysis of Hypertension -- Tissue Proteomics in Vascular Disease -- Urine Metabolomics in Hypertension Research -- Systems Biology Approach in Hypertension Research -- Measurement of Angiotensin Peptides: HPLC, RIA -- Measurement of Angiotensin Converting Enzyme 2 Activity in Biological Fluid (ACE2) -- Determining the Enzymatic Activity of Angiotensin-Converting Enzyme 2 (ACE2) in Brain Tissue and Cerebrospinal Fluid Using a Quenched Fluorescent Substrate -- Measurement of Cardiac Angiotensin II by Immunoassays, HPLC-chip/Mass Spectrometry and Functional Assays -- Analysis of the Aldosterone Synthase (CYP11B2) and 11b-Hydroxylase (CYP11B1) Genes -- Dopaminergic Immunofluorescence Studies in Kidney Tissue -- Techniques for the Evaluation of the Genetic Expression, Intracellular Storage and Secretion of Polypeptide Hormones with Special Reference to the Natriuretic Peptides (Nps) -- Intracellular Free Calcium Measurement Using Confocal Imaging -- Measuring T-type Calcium Channel Currents in Isolated Vascular Smooth Muscle Cells -- In Vitro Analysis of Hypertensive Signal Transduction: Kinase Activation, Kinase Manipulation, and Physiologic Outputs -- In vitro and in vivo Approaches to Assess Rho-kinase Activity -- NADPH Oxidases and Measurement of Reactive Oxygen Species -- Measurement of Superoxide Production and NADPH Oxidase Activity by HPLC Analysis of Dihydroethidium Oxidation -- Assessment of Caveolae/Lipid Rafts in Isolated Cells -- Isolation and Characterization of Circulating Microparticles by Flow Cytometry -- Isolation of Mature Adipocytes from White Adipose Tissue and Gene Expression Studies by Real-Time Quantitative RT-PCR -- Isolation and Differentiation of Murine Macrophages -- Isolation and Differentiation of Human Macrophages -- Isolation of Immune Cells for Adoptive Transfer -- Isolation and Culture of Endothelial Cells from Large Vessels -- Isolation and Culture of Vascular Smooth Muscle Cells from Small and Large Vessels -- Evaluation of Endothelial Dysfunction in vivo -- Vascular Reactive of Isolated Aorta To Study the Angiotensin-(1-7) Actions -- Generation of a Mouse Model with Smooth Muscle Cell Specific Loss of the Expression of< PPARγ -- Renal Delivery of Anti-microRNA Oligonucleotides in Rats -- In Vivo Analysis of Hypertension: Induction of Hypertension, In Vivo Kinase Manipulation And Assessment Of Physiologic Outputs. .
Keywords: Molecular biology; Molecular Medicine