Herpes Simplex Virus : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2020.
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| Edición: | 2nd ed. 2020. |
| Colección: | Methods in Molecular Biology,
2060 |
| Materias: |
Tabla de Contenidos:
- Tour de Herpes: Cycling through the Life and Biology of HSV-1
- Vaccines for Herpes Simplex: Recent Progress Driven by Viral and Adjuvant Immunology
- Herpes Simplex Virus Growth, Preparation, and Assay
- Engineering HSV-1 Vectors for Gene Therapy
- Preparation of Herpes Simplex Virus Type 1 (HSV-1)-Based Amplicon Vectors
- HSV-1 Amplicon Vectors as Genetic Vaccines
- oHSV Genome Editing by Means of galK Recombineering
- Rescue, Purification, and Characterization of a Recombinant HSV Expressing a Transgenic Protein
- CRISPR/Cas9-Based Genome Editing of HSV
- Latent/Quiescent Herpes Simplex Virus 1 Genome Detection by Fluorescent In Situ Hybridization (FISH)
- Oligonucleotide Enrichment of HSV-1 Genomic DNA from Clinical Specimens for use in High-Throughput Sequencing
- HSV Mutant Generation and Dual-Detection Methods for Gaining Insight into Latent/Lytic Cycles In Vivo
- Phenotypic and Genotypic Testing of HSV-1 and HSV-2 Resistance to Antivirals
- Using Primary SCG Neuron Cultures to Study Molecular Determinants of HSV-1 Latency and Reactivation
- Characterization of Extracellular HSV-1 Virions by Proteomics
- Analysis and Sorting of Individual HSV-1 Particles by Flow Virometry
- Isolation/Analysis of Extracellular Microvesicles from HSV-1-Infected Cells
- Conformational Change in Herpes Simplex Virus Entry Glycoproteins Detected by Dot Blot
- BioID Combined with Mass Spectrometry to Study Herpesvirus Protein-Protein Interaction Networks
- Preparation of Herpes Simplex Virus Infected Primary Neurons for Transmission Electron Microscopy
- Transmission Immuno-Electron Microscopy of Herpes Simplex Virus-1 Infected Dorsal Root Ganglia Neurons Sectioned in Growth Plane
- Multi-Fluorescence Live Analysis of Herpes Simplex Virus Type-1 Replication
- Expression, Purification, and Crystallization of HSV-1 Glycoproteins for Structure Determination
- Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination
- The Use of Microfluidic Neuronal Devices to Study the Anterograde Axonal Transport of Herpes Simplex Virus-1
- A Model of In Vivo HSV-1 DNA Transport using Murine Retinal Ganglion Cells
- The Murine Intravaginal HSV-2 Challenge Model for Investigation of DNA Vaccines.