Heterologous Protein Production in CHO Cells : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Meleady, Paula. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1603
Materias:
Tabla de Contenidos:
  • Strategies and Considerations for Improving Expression of 'Difficult to Express' Proteins in CHO Cells
  • Glycoengineering of CHO Cells to Improve Product Quality
  • Large-Scale Transient Transfection of Chinese Hamster Ovary Cells in Suspension
  • Cloning of Single-Chain Antibody Variants by Overlap-Extension PCR for Evaluation of Antibody Expression in Transient Gene Expression
  • Anti-Apoptosis Engineering for Improved Protein Production from CHO Cells
  • Conditional Knockdown of Endogenous MicroRNAs in CHO Cells using TET-ON-SanDI Sponge Vectors
  • Application of CRISPR/Cas9 Genome Editing to Improve Recombinant Protein Production in CHO Cells
  • Improved CHO Cell Line Stability and Recombinant Protein Expression During Long-Term Culture
  • Selection of High-Producing Clones using FACS for CHO Cell Line Development
  • The 'Omics Revolution in CHO Biology: Roadmap to Improved CHO Productivity
  • A Bioinformatics Pipeline for the Identification of CHO Cell Differential Gene Expression from RNA-Seq Data
  • Filter-Aided Sample Preparation (FASP) for Improved Proteome Analysis of Recombinant Chinese Hamster Ovary Cells
  • Phosphopeptide Enrichment and LC-MS/MS Analysis to Study the Phosphoproteome of Recombinant Chinese Hamster Ovary Cells
  • Engineer Medium and Feed for Modulating N-Glycosylation of Recombinant Protein Production in CHO Cell Culture
  • Glycosylation Analysis of Therapeutic Glycoproteins Produced in CHO Cells
  • Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses.