MicroRNAs and the Immune System : Methods and Protocols - Monticelli, Silvia; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2010)
Analysis of miRNA Expression: Classic Methods Revisited -- A Rapid, Quantitative Assay for Direct Detection of MicroRNAs and Other Small RNAs Using Splinted Ligation -- Normalization of MicroRNA Quantitative RT-PCR Data in Reduced Scale Experimental Designs -- MicroRNA Detection in Bone Marrow Cells by LNA-FISH -- Measuring MicroRNA Expression in Size-Limited FACS-Sorted and Microdissected Samples -- High-Throughput Analysis of miRNAs -- MicroRNA Cloning from Cells of the Immune System -- High-Throughput Profiling in the Hematopoietic System -- Construction of Small RNA cDNA Libraries for Deep Sequencing -- MicroRNA-Profiling in Formalin-Fixed Paraffin-Embedded Specimens -- Functional Analysis of miRNAs in the Immune System: Gain-of-Function -- Expression of miRNAs in Lymphocytes: A Review -- Mouse Models for miRNA Expression: The ROSA26 Locus -- Regulation of Monocytopoiesis by MicroRNAs -- MicroRNA Activity in B Lymphocytes -- Isolation and Characterization of MicroRNAs of Human Mature Erythrocytes -- Stable Overexpression of miRNAs in Bone Marrow-Derived Murine Mast Cells Using Lentiviral Expression Vectors -- Monitoring MicroRNA Activity and Validating MicroRNA Targets by Reporter-Based Approaches -- Functional Analysis of miRNAs in the Immune System: Loss-of-Function -- Lentivirus-Mediated Antagomir Expression -- miRNA Post-Transcriptional Modifications and Mechanisms of Action -- Solution Structure of miRNA:mRNA Complex -- MiRNA Editing -- Bioinformatic Analysis and Target Prediction -- Computational Prediction of MicroRNA Targets -- Large-Scale Integration of MicroRNA and Gene Expression Data for Identification of Enriched MicroRNA-mRNA Associations in Biological Systems -- miRNA and Viruses -- Identification and Validation of the Cellular Targets of Virus-Encoded MicroRNAs.
Keywords: Immunology; Immunology