HIV Protocols : Second Edition - Prasad, Vinayaka R.; Kalpana, Ganjam V.; SpringerLink (Online service) (Totowa, NJ : Humana Press : Imprint: Humana, 2009)
Preparation of Virus Particles and their Analysis -- Methods for Viral RNA Isolation and PCR Amplification for Sequencing of Near Full-Length HIV-1 Genomes -- Purification of HIV-1 Virions by Subtilisin Digestion or CD45 Immunoaffinity Depletion for Biochemical Studies -- Calculating HIV-1 Infectious Titre Using a Virtual TCID50 Method -- Methods To Study Hiv-1 Replication -- Cell-Free Assays for HIV-1 Uncoating -- Real-Time PCR Analysis of HIV-1 Replication Post-entry Events -- Analysis of 2-LTR Circle Junctions of Viral DNA in Infected Cells -- HIV-1 Recombination: An Experimental Assay and a Phylogenetic Approach -- Methods of Preparation and Analysis of Intracellular Reverse Transcription Complexes -- Analysis of Viral and Cellular Proteins in HIV-1 Reverse Transcription Complexes by Co-immunoprecipitation -- Isolation and Analysis of HIV-1 Preintegration Complexes -- Bisarsenical Labeling of HIV-1 for Real-Time Fluorescence Microscopy -- Methods for the Study of HIV-1 Assembly -- Assembly of Immature HIV-1 Capsids Using a Cell-Free System -- Preparation of Recombinant HIV-1 Gag Protein and Assembly of Virus-Like Particles In Vitro -- Methods for the Analysis of HIV-1 Nucleocapsid Protein Interactions with Oligonucleotides -- Methods for Analysis of Incorporation and Annealing Methods for Analysis of tRNALys in HIV-1 -- Specialized Approaches To Study Hiv-1 Biology and Pathogenesis -- Somatic Cell Genetic Analyses to Identify HIV-1 Host Restriction Factors -- Rapid, Controlled and Intensive Lentiviral Vector-Based RNAi -- Reverse Two-Hybrid Screening to Analyze Protein-Protein Interaction of HIV-1 Viral and Cellular Proteins -- Methods to Study Monocyte Migration Induced by HIV-Infected Cells -- Novel Mouse Models for Understanding HIV-1 Pathogenesis -- Immunological Studies Of Hiv -- Mucosal Antibody Responses to HIV -- Isolating Mucosal Lymphocytes from Biopsy Tissue for Cellular Immunology Assays -- Quantifying HIV-1-Specific CD8 + T-Cell Responses Using ELISPOT and Cytokine Flow Cytometry -- Multiparameter Flow Cytometry Monitoring of T Cell Responses -- Measuring HIV Neutralization in a Luciferase Reporter Gene Assay -- Assessing the Antiviral Activity of HIV-1-Specific Cytotoxic T Lymphocytes -- Methods for Quantitating Antigen-Specific T Cell Responses Using Functional Assays in Rhesus Macaques -- Drug Resistant Viruses And Viral Evolution -- Isolation of Drug-Resistant Mutant HIV Variants Using Tissue Culture Drug Selection -- Virus Evolution as a Tool to Study HIV-1 Biology.
Keywords: Infectious diseases; Immunology; Virology; Infectious Diseases; Immunology; Virology