Microbial Steroids : Methods and Protocols - Barredo, José-Luis.; Herráiz, Ignacio; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2017)
Steroid Bioconversions -- Chemical Pathways of Corticosteroids, Industrial Synthesis from Sapogenins -- Genome Sequencing of Steroid Producing Bacteria with Illumina Technology -- Genome Sequencing of Steroid Producing Bacteria using Ion Torrent Technology and a Reference Genome -- RNA-Seq-Based Comparative Transcriptomics: RNA Preparation and Bioinformatics -- Intra- and Extra-Cellular Proteome Analyses of Steroid-Producer Mycobacteria -- Genetic Techniques for Manipulation of the Phytosterol Biotransformation Strain Mycobacteriumneoaurum NRRL B-3805 -- Identification and Characterization of the genes and Enzymes Belonging to the Bile Acid Catabolic Pathway in Pseudomonas -- Selection of Biodegrading Phytosterol Strains -- Stigmasterol Removal by an Aerobic Treatment System -- Production and Biotransformation of Phytosterol Microdispersions to Produce 4-Androstene-3, 17-Dione -- Β-Sitosterol Bioconversion to Androstenedione in Microtiter Plates -- Bioconversion of Phytosterols into Androstenedione by Mycobacterium -- Scale-Up of Phytosterols Bioconversion into Androstenedione -- Bioconversion of Phytosterols into Androstadienedione by Mycobacterium smegmatis CECT 8331 -- Process for Biotransformation of Androsta-4-ene-3, 17-Dione (4-AD) to Androsta-1, 4-Diene-3, 17-Dione (ADD) -- Laboratory-Scale Hydroxylation of Steroids by P450BM3 Variants -- Obtaining of 11α-Hydroxy and Rost-4-ene-3, 17-Dione from Natural Sterols -- Steroid 11-Alfa-Hydroxylation by the Fungi Aspergillus nidulans and Aspergillus ochraceus -- Biotransformation of DHEA into 7α, 15α-diOH-DHEA -- Stabilization of Enzymes by Using Thermophiles -- Biocatalysis of Steroids with Mycobacterium Sp. in Aqueous and Organic Media -- Downstream Process Synthesis for Microbial Steroids -- Analysis of Intermediates of Steroid Transformations in Resting Cells by Thin Layer Chromatography (TLC) -- A Ligand-Binding Assay to Measure the Affinity and Specificity of Sterol-Binding Proteins In Vitro. .
Keywords: Biochemistry; Biochemistry, general