HIV Protocols - Prasad, Vinayaka R.; Kalpana, Ganjam V.; SpringerLink (Online service) (New York, NY : Springer New York : Imprint: Humana, 2016)
Quantifying CD4/CCR5 Usage Efficiency of HIV-1 ENV Using the Affinofile System -- Measuring T Cell-to-T Cell HIV-1 Transfer, Viral Fusion, and Infection Using Flow Cytometry -- HIV-1 Capsid Stabilization Assay -- Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity -- HIV-1 Reverse Transcriptase Based Assay to Determine Cellular dNTP Concentrations -- Rapid Determination of HIV-1 Mutant Frequencies and Mutation Spectra Using an mCherry/EGFP Dual Reporter Viral Vector -- Novel Biochemical Tools for Probing HIV RNA Structure -- Analysis of HIV-1 Gag-RNA Interactions in Cells and Virions by CLIP-seq -- Isolation of Cognate Cellular and Viral Ribonucleoprotein Complexes of HIV-1 RNA Applicable to Proteomic Discovery and Molecular Investigations -- Methods for the Analyses of Inhibitor-Induced Aberrant Multimerization of HIV-1 Integrase -- Quantification of HIV-1 Gag Localization Within Virus Producer Cells -- Methods to Study Determinants for Membrane Targeting of HIV-1 Gag In Vitro -- Visualizing the Behavior of HIV-Infected T Cells In Vivo Using Multiphoton Intravital Microscopy -- Modeling HIV-1 Mucosal Transmission and Prevention in Humanized Mice -- High Throughput Humanized Mouse Models for Evaluation of HIV-1 Therapeutics and Pathogenesis -- Measuring the Frequency of Latent HIV-1 in Resting CD4+ T Cells Using a Limiting Dilution Co-Culture Assay -- LGIT In Vitro Latency Model in Primary and T Cell Lines to Test HIV-1 Reactivation Compounds -- Improved Methods to Detect Low Levels of HIV Using Antibody-Based Technologies -- Analysis of ABCA1 and Cholesterol Efflux in HIV-Infected Cells -- The Proteomic Characterization of Plasma or Serum from HIV-Infected Patients -- Proteomic Characterization of Exosomes from HIV-1-Infected Cells -- Detecting HIV-1 Tat in Cell Culture Supernatants by ELISA or Western Blot -- Protocol for Detection of HIV-Tat Protein in Cerebrospinal Fluid by a Sandwich Enzyme Linked Immunosorbent Assay -- Measuring the Uptake and Transactivation Function of HIV-1 Tat Protein in a Trans-Cellular Co-Cultivation Set Up -- Evaluating the Role of Viral Proteins in HIV-Mediated Neurotoxicity Using Primary Human Neuronal Cultures.
Keywords: Immunology; Virology; Immunology; Virology